JAK2 notes

Deep research (falcon / perplexity-lite) failed (providers unavailable). Notes from cached publications and UniProt O60674.

Core biology

• Non-receptor tyrosine kinase; FERM-SH2 region binds cytokine receptor box1/box2; JH2 pseudokinase (regulatory, weak dual-specificity kinase PMID:21841788); JH1 kinase.
• Activation loop Y1007 essential PMID:9111318.
• EPO signaling in primary human erythroid cells PMID:9657743.
• beta c association PMID:8007942; IL-23R PMID:12023369; gp130/LIFR PMID:8272873.
• FERM domain/box1-box2 needed for TpoR surface expression and stability PMID:15899890.
• Knockout: no definitive erythropoiesis, no IFN-gamma response PMID:9590174.
• Nuclear H3Y41 phosphorylation PMID:19783980 -- treated as non-core.

Localization

Cytoplasmic, peripheral at the inner (cytoplasmic) face of the plasma membrane via receptor binding; no TM, no
extracellular exposure. Also endomembranes during receptor trafficking, and a reported nuclear pool.
Reactome "endosome lumen" assignment is topologically impossible -> REMOVE.

Curation approach

396 GOA rows (159 Reactome cytosol rows). Core: PTK activity, cytokine receptor binding, JAK-STAT and specific
cytokine pathways. Downstream physiology KEEP_AS_NON_CORE; apoptosis machinery / response-to-chemical / adhesion
machinery terms marked over-annotated; generic protein binding removed except receptor partners (-> cytokine receptor binding).