Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Atomic-level models of the bacterial carboxysome shell.
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Synechocystis CcmL (and alpha-carboxysomal CsoS4A) form pentamers compatible with the vertices of an icosahedral shell.
"Both proteins assemble to form pentameric structures whose size and shape are compatible with formation of vertices in an icosahedral shell."
A multiprotein bicarbonate dehydration complex essential to carboxysome function in cyanobacteria.
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In Synechocystis, CcmL interacts (yeast two-hybrid) with CcmM73, which links the bicarbonate dehydration complex to the shell; the interaction requires both CcmM73 domains.
"Interactions between CcmM73 and CcmK and between CcmM73 and CcmL required both the N-terminal and the C-terminal domains of the CcmM73 polypeptide"
Analysis of a genomic DNA region from the cyanobacterium Synechococcus sp. strain PCC7942 involved in carboxysome assembly and function.
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Insertional inactivation of ccmL in S. elongatus PCC 7942 gives a high-CO2-requiring mutant with rod-shaped carboxysomes.
"The PVU mutant has a drug marker inserted into the ccmL gene, and it possesses abnormal rod-shaped carboxysomes."
Structural determinants of the outer shell of β-carboxysomes in Synechococcus elongatus PCC 7942: roles for CcmK2, K3-K4, CcmO, and CcmL.
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CcmK2, CcmO and CcmL are the minimal structural determinants of the beta-carboxysome outer shell, with CcmL at the vertices.
"These data suggested a model for the facet structure of β-carboxysomes with CcmL forming the vertices"
The pentameric vertex proteins are necessary for the icosahedral carboxysome shell to function as a CO2 leakage barrier.
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Loss of alpha-carboxysome vertex pentamers (CsoS4A/B, Halothiobacillus) makes the shell leaky to CO2 without abolishing shell formation.
"In the absence of the CsoS4 proteins the carboxysome shell loses its limited permeability to CO(2)"
Biogenesis of a bacterial organelle: the carboxysome assembly pathway.
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Beta-carboxysome shell proteins (hexamers and pentamers) encapsulate a pre-assembled cargo core.
"Subsequently, shell proteins encapsulate this procarboxysome, inducing budding and distribution of functional organelles within the cell."
Two new high-resolution crystal structures of carboxysome pentamer proteins reveal high structural conservation of CcmL orthologs among distantly related cyanobacterial species.
Interactions and structural variability of β-carboxysomal shell protein CcmL.
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CcmL interacts only weakly with CcmK2 in vitro, suggesting it joins the shell at defect points.
"this would support the idea that CcmL can only interact with CcmK2 at rare defect points in the growing shell"
UniProtKB entry P72759 (CCML_SYNY3)