Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa.
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
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mexEF-oprN is positively regulated by an upstream LysR-family protein (MexT).
"Expression of the mexEF-oprN operon was shown to be positively regulated by a protein encoded on a 1.5 kb DNA fragment located upstream of mexE"
Transcriptional regulation of the mexEF-oprN multidrug efflux pump operon by MexT and an unidentified repressor in nfxC-type mutant of Pseudomonas aeruginosa.
Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa.
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MexT directly regulates a conserved-motif regulon independent of MexEF-OprN.
"Thus, a novel regulon directly modulated by MexT, that includes but is independent of mexEF-oprN, has been identified."
Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa.
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DNase I footprinting identifies two MexT sites in the mexEF-oprN promoter.
"A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF oprN promoter."
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Full-length MexT is a homotetramer.
"FL MexT and MexT RD were homotetrameric and homodimeric in solution, respectively, as determined using SEC-MALS"
MexT regulates the type III secretion system through MexS and PtrC in Pseudomonas aeruginosa.
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A PAO1 mexT mutant secretes more ExoS.
"However, a mexT mutant of PAO1 (PAO1 mexT ::Gm) resulted in much higher ExoS secretion than that of its parental strain PAO1"
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mexT and mexS mutants make more elastase and pyocyanin.
"the mexS and mexT mutants of PAO1 shared the same phenotype of increased elastase and pyocyanin production compared to that in wild-type PAO1"
Mutations in mexT bypass the stringent response dependency of virulence in Pseudomonas aeruginosa.
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Loss of MexT raises aminoglycoside resistance.
"leads to elevated aminoglycoside resistance"
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MexT is an activator of mexEF-oprN.
"However, MexT is an activator of mexEF-oprN expression"
Modulation of the Pseudomonas aeruginosa quorum sensing cascade by MexT-regulated factors.
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MexT represses PQS signalling and pqsE through MexEF-OprN.
"we hypothesized that constitutive MexT activity induces mexEF-oprN expression, leading to the repression of PQS QS, delayed pqsE expression, and delayed RhlR activation."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees