Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning and characterization of a human polyamine oxidase that is inducible by polyamine analogue exposure.
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Cloning of human PAOh1/SMOX; the cDNA product has FAD-dependent polyamine oxidase activity inhibited by the specific mammalian PAO inhibitor MDL 72,527 but not by monoamine- or diamine-oxidase inhibitors, and is inducible by antitumor polyamine analogues.
Identification and characterization of a novel flavin-containing spermine oxidase of mammalian cell origin.
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Identifies SMOX as a flavin-containing spermine oxidase that strongly favors spermine over N1-acetylspermine and does not act on N1-acetylspermidine, spermidine or N1,N12-diacetylspermine, defining the direct (non-acetylation-dependent) spermine oxidase activity.
Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics.
Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion.
Nuclear localization of human spermine oxidase isoforms - possible implications in drug response and disease etiology.
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Demonstrates nuclear localization of SMO/PAOh1 and the SMO5 splice variant by confocal microscopy, with increased nuclear spermine oxidation producing H2O2 near DNA, linking SMOX to oxidative DNA damage and inflammation-induced carcinogenesis.
PAOs oxidise polyamines to amines
SMOX-3 oxidises SPN to SPM
Interconversion of polyamines