Affinage mechanistic annotation for TAC1 (human) Affinage Affinage (Claude Sonnet reading pass + Opus synthesis pass) 36 citations

Affinage mechanistic annotation for TAC1 (human)

Current model (mechanistic narrative)

The TAC1 symbol in this corpus resolves to at least three biologically unrelated genes, and the mammalian TAC1 entries describe a tachykinin neuropeptide precursor. In mammals, TAC1 encodes substance P and neurokinin A, neuropeptides whose loss diminishes anxiety- and depression-related behavior and disrupts stress and fear responses PMID:12427862. These peptides act largely through NK1R: Tac1-expressing neurons drive defined circuits including a lateral/ventrolateral PAG pathway that facilitates the itch-scratching cycle via spinal GRPR neurons PMID:30554781, a nucleus accumbens shell projection to ventral pallidum that bidirectionally controls stress-induced anhedonia PMID:33147466, and an accumbens medial-shell projection to lateral hypothalamus governing aversive avoidance PMID:36901777. TAC1 tachykinins are required for injury-induced nociceptor mechanical sensitization and hyperexcitability PMID:31012376, for morphine-induced respiratory depression and opioid withdrawal aversion PMID:20590634, and they support endogenous spinal opioid peptide tone PMID:26072188. Beyond the nervous system, TAC1 peptides maintain basal cutaneous microcirculation PMID:23499760, modulate puberty and GnRH-neuron responsiveness to kisspeptin via NK1R-Kiss1R heterodimerization PMID:28444173, control energy balance and circadian feeding through the melanocortin/POMC system PMID:28775376, and act as prolactin secretagogues in the seasonally regulated pars tuberalis PMID:20434341. TAC1 transcription is repressed by REST, which cooperates synergistically with NFκB at sites within exon 1 [PMID:19246391, PMID:17709376], and is activated downstream of SDF-1α through CRE/CREB signaling using distinct pathways in different cell types—cAMP-independent Gαi2-PI3K-PKCζ-ERK signaling in breast cells PMID:18316470—together with JNK/ATF-2/AP-1 input and enhancer (ECR) synergy in differentiating and sensory neurons [PMID:21160161, PMID:21671725], plus MeCP2 binding and promoter CpG methylation PMID:23759142. Separately and unrelated to the neuropeptide gene, the C. elegans TAC-1 (a TACC-family microtubule-associated protein) and the Candida/Candida parapsilosis Tac1 (a zinc-cluster transcription factor) and a plant TAC1 (tiller angle control) appear in this corpus as symbol collisions describing entirely distinct proteins.

Affinage mechanism profile (Affinage's own GO/Reactome grounding)

Dated findings (citation-anchored)

Year Confidence Finding PMIDs Journal
2003 High TAC-1 (C. elegans TACC protein) physically interacts with ZYG-9 (XMAP215 family MAP) both in vitro and in vivo, and the two proteins mutually stabilize each other; TAC-1 centrosomal localization requires gamma-tubulin and Aurora-A kinase AIR-1 activity; loss of TAC-1 results in defective microtubule assembly as measured by FRAP. PMID:12956950, PMID:12956951, PMID:12956952 Current biology : CB
2003 High TAC-1 depletion (RNAi) in C. elegans embryos produces very short centrosomal microtubules and short spindles without reducing alpha-tubulin intensity near centrosomes, indicating microtubule nucleation is intact but elongation/stabilization is impaired; TAC-1 and ZYG-9 are mutually dependent for centrosomal localization. PMID:12956951, PMID:12956952 Current biology : CB
2007 High In C. elegans, TAC-1 physically interacts with ZYG-8 (doublecortin domain protein) through ZYG-8's doublecortin domain; TAC-1 and ZYG-8 form a complex that does not contain ZYG-9 in vivo; TAC-1 is required for correct ZYG-9 centrosomal enrichment; the ZYG-9-TAC-1 complex is required for correct anaphase spindle positioning. PMID:17666432 Journal of cell science
2023 Medium In C. elegans, Shugoshin SGO-1 binds TAC-1 (TACC protein) and constrains TAC-1 to the ciliary basal body via the transition zone; TAC-1 activity must be maintained below a threshold at the ciliary base for correct cilia function, and SGO-1 participates in this regulation. PMID:37296204 Scientific reports
2002 High Targeted deletion of the Tac1 gene (encoding substance P and neurokinin A) in mice diminishes anxiety- and depression-related behaviors, establishing that Tac1-encoded tachykinins are required for normal fear and stress responses. PMID:12427862 The Journal of neuroscience : the official journal of the Society for Neuroscience
2018 High Tac1-expressing glutamatergic neurons in the lateral/ventrolateral PAG facilitate the itch-scratching cycle via descending regulation; ablation or suppression of these neurons decreases itch-induced scratching; their activation-evoked scratching is suppressed by ablation of spinal GRPR neurons. PMID:30554781 Neuron
2014 High Tac2 gene (encoding NkB) expressed in centromedial amygdala (CeM) neurons is required for fear memory consolidation; NkB and its receptor Nk3R mediate this effect; increased Tac2 expression or lentiviral CeA overexpression enhances fear consolidation, blocked by Nk3R antagonist osanetant; silencing Tac2-expressing neurons via DREADDs impairs fear consolidation. PMID:24976214 Neuron
2016 Medium In vivo optogenetic stimulation of CeA Tac2-expressing neurons (ChR2 knockin mice) during fear acquisition enhanced fear memory consolidation and drove action potential firing in vitro; Tac2-CeA neurons co-express striatal-enriched protein tyrosine phosphatase (STEP), which may regulate Nk3R signaling. PMID:27238620 Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
2018 High Chronic social isolation stress (2 weeks) in mice induces brain-wide upregulation of Tac2/NkB; systemic Nk3R antagonist prevents virtually all behavioral effects of chronic social isolation; enhancing NkB expression and release in group-housed mice phenocopies social isolation stress; dissociable region-specific requirements for Tac2 peptide and Nk3R in different behavioral changes. PMID:29775595 Cell
2021 Medium Sex differences exist in Tac2 pathway regulation of fear memory consolidation: CeA-Tac2 antagonism impairs fear memory in males but enhances it in females; CeA-testosterone mediates Tac2 effects in males, CeA-estradiol in females; Akt/GSK3β/β-Catenin signaling mediates the sex-differential Tac2 pathway regulation. PMID:33941789 Nature communications
2020 Medium Tac1-expressing neurons in the nucleus accumbens (NAc) lateral shell project to ventral pallidum and contribute to stress-induced anhedonia-like behavior; selective inhibition and activation of Tac1-NAc neurons bidirectionally modulate stress susceptibility; inhibition of neurokinin 1 receptor promotes susceptibility to social stress. PMID:33147466 Cell reports
2023 Medium Tac1 neurons in the NAc medial shell project to the lateral hypothalamic area (LH); the NAc-Tac1→LH pathway contributes to avoidance responses to aversive stimuli; medial prefrontal cortex sends excitatory inputs to NAc-Tac1 neurons regulating avoidance. PMID:36901777 International journal of molecular sciences
2009 Medium REST (RE-1 silencing transcription factor) binds the 5' UTR of the TAC1 promoter and suppresses TAC1 expression; REST expression in breast cancer cells is inversely proportional to aggressiveness; REST knockdown increases TAC1 expression, proliferation, and migration in low-metastatic cells; ectopic REST in aggressive cells reduces these parameters. PMID:19246391 Proceedings of the National Academy of Sciences of the United States of America
2007 Medium REST and NFκB synergistically repress TAC1 transcription in human mesenchymal stem cells; two REST-binding sites are adjacent to one NFκB site within exon 1 of the TAC1 promoter; ChIP and mutagenesis confirmed both factors cooperate for repression in neurogenic and cytokine-stimulated conditions. PMID:17709376 The Journal of biological chemistry
2010 Medium TAC1 promoter requires synergy with a remote enhancer element (ECR2) to respond to MEK/ERK (MAPK) signaling in sensory neurons; antagonism of the MEK pathway blocks noxious stimulation-driven TAC1 enhancer-promoter synergy; capsaicin induction involves a non-cell-autonomous mechanism in larger diameter neurons. PMID:21160161 Neuro-Signals
2011 Medium NK1 receptor is expressed in all SP-expressing sensory neurons after capsaicin induction; an NK1 agonist activates both SP expression and the TAC1 ECR1 enhancer-promoter in larger diameter neurons, demonstrating an autocrine loop controlling TAC1 promoter activity in sensory neurons. PMID:21294877 Journal of neuroinflammation
2011 Medium During MSC-to-neuron differentiation, CRE1 and CRE2/AP-1 sites in the TAC1 promoter are activated sequentially (days 6 and 12); decrease of REST activates JNK, which activates ATF-2 and AP-1 to bind CRE1 and CRE2/AP-1 respectively; JNK inhibition blocks TAC1 induction; transplanted JNK-pathway-active MSCs improve spinal cord injury in zebrafish. PMID:21671725 Stem cells and development
2007 Medium SDF-1α regulates TAC1 expression in bone marrow stromal cells via NF-κB; at high SDF-1α levels (≥50 ng/mL) NF-κB activation mediates repression of TAC1 within exon 1; substance P produced downstream does not regulate SDF-1α production (negative finding); substance P signals through NK1 (not NK2) receptor to stimulate hematopoiesis. PMID:17277111 Journal of immunology (Baltimore, Md. : 1950)
2008 Medium In non-tumorigenic breast cells, SDF-1α at low concentrations activates TAC1 via CRE sites through a non-canonical Gαi2-PI3K-PKCζ-p38-ERK pathway to phosphorylate CREB, distinct from the cAMP-PKA pathway used in bone marrow stromal cells. PMID:18316470 Journal of molecular endocrinology
2013 Medium MeCP2 binds directly to the TAC1 promoter in HEK cells as shown by ChIP; antiepileptic drug (valproic acid) treatment alters MeCP2 binding to TAC1 promoter; TAC1 promoter CpG hypermethylation correlates with reduced TAC1 expression in autism and seizure disorder. PMID:23759142 Journal of neurodevelopmental disorders
2007 Low Post-transcriptional regulation of Tac1 mRNA in bone marrow stroma involves RNA-binding proteins (with differential binding kinetics for stimulatory vs. inhibitory cytokines) and cytokine-induced miRNAs that interact with the 3' UTR of Tac1 mRNA. PMID:18061399 Brain, behavior, and immunity
2010 Medium Tac1-encoded tachykinins (substance P and neurokinin A) are required for morphine-induced respiratory depression and the aversive aspect of opioid withdrawal; in Tac1-/- mice morphine analgesia is enhanced and behavioral sensitization (addiction mechanism) is reduced. PMID:20590634 British journal of pharmacology
2017 Medium Tac1 knockout male mice show delayed puberty, decreased Pdyn and Nos1 expression, and elevated GnRH levels; kisspeptin receptor (Kiss1R) and substance P receptor (NK1R) heterodimerize, suggesting SP tone alters GnRH neuron responsiveness to kisspeptin; Tac1-/- mice show decreased LH response to central kisspeptin and senktide administration despite intact GnRH neuron stimulation. PMID:28444173 Endocrinology
2017 Medium TAC1-encoded substance P and neurokinin A maintain basal cutaneous microcirculation; Tac1-/- mice show significantly lower basal postoperative skin microcirculation but mustard oil-induced neurogenic vasodilation is unaffected, while motor coordination is impaired in Tac1-/- mice. PMID:23499760 Peptides
2019 Medium Tac1 knockout nociceptors display disrupted encoding of tonic suprathreshold mechanical stimuli and fail to develop mechanical sensitization after injury; Tac1-/- mice show reduced paw edema, hypersensitivity, and weight-bearing deficits after incision, and their nociceptors lack post-incision electrical hyperexcitability, despite normal CGRP upregulation. PMID:31012376 Molecular pain
2015 Medium Absence of Tac1-encoded tachykinins (Tac1-/- mice) is associated with significantly lower spinal cord concentrations of opioid peptides endomorphin-2, leucine-enkephalin, and dynorphin A (Dyn A ~3-fold lower), suggesting tachykinin system supports endogenous opioid tone. PMID:26072188 Neuropeptides
2017 Medium Tac1 ablation in mice produces a lean phenotype with reduced food intake, altered circadian feeding rhythm (disrupted clock gene Cry1/2, Per1/2 expression in SCN, MBH, and liver), increased proopiomelanocortin (POMC) expression in MBH, resistance to diet-induced obesity, and improved glucose tolerance; Tac1 controls energy balance through the melanocortin system. PMID:28775376 International journal of obesity (2005)
2017 Medium In Candida albicans, hyperactivated Tac1 (by gain-of-function mutations or xenobiotics) facilitates recruitment of the Mediator coactivator complex to the CDR1 promoter; CDR1 activation and azole resistance depend on the Tac1 C-terminal transcriptional activation domain (TAD) and Mediator tail module subunits; Tac1 hyperactivation correlates with Mediator-dependent Tac1 phosphorylation; the Tac1 middle region negatively regulates the TAD. PMID:28807920 Antimicrobial agents and chemotherapy
2023 Medium In C. albicans Tac1, the N-terminal DNA binding domain (DBD) interacts with the Drug Responsive Element (DRE) in CDR1/CDR2 promoters; the C-terminal Acidic Activation Domain (AAD) interacts with TATA box binding protein (TBP); the Middle Homology Region (MHR) acts as a xenobiotic binding domain (XBD) important for drug resistance. PMID:37502396 Frontiers in microbiology
2018 Medium C. albicans Tac1 and Znc1 are functionally activated by farnesol (quorum-sensing molecule) and together bind the CDR1 promoter to upregulate CDR1; CDR1 expression then facilitates farnesol efflux; Tac1 and Znc1 have overlapping but non-identical regulons. PMID:30104273 Antimicrobial agents and chemotherapy
2023 High In Candida parapsilosis, gain-of-function mutation CpTac1-G650E causes 8-fold increase in fluconazole MIC and overexpression of CpCDR1, CpCDR1B, and CpCDR1C; correction of this mutation reduces MIC 16-fold; disruption of CDR1/CDR1B/CDR1C together reduces MIC 4-fold, establishing CpTac1 as a direct regulator of efflux pump-mediated triazole resistance. PMID:37666448 Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
2010 Medium TAC1 is expressed in the sheep pars tuberalis (PT) and strongly activated by long photoperiod; TAC1-encoded peptides (substance P and neurokinin A) act as prolactin secretagogues on primary pituitary cells, making TAC1 a candidate for the PT-expressed 'tuberalin' seasonal hormone regulator. PMID:20434341 Current biology : CB
2025 Low In indica rice, fine-tuning TAC1 expression levels via CRISPR-Cas9 editing of upstream and downstream non-coding regions produces gradient tiller angle changes proportional to TAC1 expression levels, with no effect on other agronomic traits; TAC1 is conserved across species including fruit trees. PMID:40052456 Journal of integrative plant biology
2024 Medium In the spinal cord dorsal horn, Tac2-expressing neurons receive direct inhibitory input from Npy neurons; during chronic itch, Y1R expression on Tac2 neurons is reduced and NPY-Y1R inhibitory regulation of Tac2 neurons is diminished, contributing to mechanical hyperknesis. PMID:38164144 Theranostics
2025 Medium PBNTac1 neurons form close synaptic connections with CeATac1 neurons; activation of the PBNTac1→CeA pathway increases scratching in histamine-induced itch but not chloroquine-induced itch; inhibition of this pathway decreases histamine-induced scratching, defining a modality-specific itch circuit. PMID:39914640 Brain research

Citations