PRS5 notes (ribose-phosphate pyrophosphokinase subunit, UniProt Q12265)

Gene-specific

• 496 aa with two NHRs; deletion reduces activity PMID:9829955
• Synthetic lethal with prs1 / prs3 PMID:9829955
• Cytoophidia in quiescence PMID:39836171 -> KEEP_AS_NON_CORE
• Adh7 interaction PMID:17892321 -> protein binding REMOVE (no functional follow-up).
• SGD IGI uses 'enables' for PRS5 but 'contributes_to' for PRS1-4; inconsistent since no subunit is active alone.

PRS family evidence (shared)

• Five PRS genes; individual subunits inactive, specific pairs active in E. coli PMID:15280369
• Active pairs PMID:15280369; stable enzyme needs >=3 subunits PMID:15280369
• Genetic architecture: PMID:10212224; Y2H PMID:10212224
• PRPP use PMID:9829955
• Conserved motifs PMID:9829955
• Localization: cytoplasm by GFP [PMID:14562095; UniProt "SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}."]
• Pairwise Prs-Prs 'protein binding' IPIs (Gavin, Betel, Tarassov, Yu, Michaelis, Uetz, Ito) all reflect hetero-oligomer assembly PMID:17892321 -> REMOVE as uninformative; captured by GO:0002189.

Curation decisions (shared)

• Core: contributes_to GO:0004749 (no subunit active alone; matches SGD IGI 'contributes_to' qualifiers), GO:0006015, cytosol, in_complex GO:0002189.
• Downstream BPs (purine nucleotide biosynthesis IBA, nucleotide biosynthesis IEA/RCA from superpathway PRPP-PWY-1, ribonucleoside monophosphate biosynthesis IEA) -> KEEP_AS_NON_CORE: PRPP is a precursor; GO:0006015 is not part_of these.
• Mg2+ binding IEA -> KEEP_AS_NON_CORE (DCbs motif).