Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E.
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Original identification of the TtgABC efflux system for toluene tolerance in DOT-T1E
"the knockedout gene was sequenced and found to be homologous to the drug exclusion gene mexB, which belongs to the efflux pump family of the resistant nodulator division type"
Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida.
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TtgR represses ttgABC expression
"In a TtgR-deficient background, expression from the ttgA promoter increased about 20-fold, suggesting that TtgR represses expression from the ttgA promoter"
Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E.
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TtgABC and TtgGHI pumps extrude aromatic solvents in DOT-T1E
"the TtgABC and TtgGHI pumps extruded toluene, styrene, m-xylene, ethylbenzene, and propylbenzene"
Mechanisms of solvent tolerance in gram-negative bacteria.
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Review of RND efflux pump mechanisms including proton motive force coupling
"The operation of these efflux pumps seems to be coupled to the proton motive force via the TonB system, although the intimate mechanism of energy transfer remains elusive"
Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance.
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Characterization of TtgB as inner membrane RND transporter across P. putida strains
"Three efflux pumps belonging to the RND (resistance-nodulation-cell division) family of multidrug extrusion pumps are the main factor involved in the high intrinsic tolerance to toluene"
Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials.
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TtgR binds phloretin and other plant antimicrobials, derepressing TtgABC expression
"TtgR represses the transcription of TtgABC, a key efflux pump in Pseudomonas putida, which is highly resistant to antibiotics, solvents and toxic plant secondary products"
Optimization of the palindromic order of the TtgR operator enhances binding cooperativity.
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TtgB possesses multidrug-binding capacity
"TtgR and the TtgB efflux pump proteins possess multidrug-binding capacity, and their concerted action is responsible for the multidrug resistance phenotype"
Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440.
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TtgABC knockout compromises survival in presence of chloramphenicol in KT2440
"survival of a knockout mutant in the TtgABC resistance-nodulation-division (RND) efflux pump and mutants in the biosynthesis of pyrroloquinoline (PQQ) were compromised in the presence of chloramphenicol"
Knockout of extracytoplasmic function sigma factor ECF-10 affects stress resistance and biofilm formation in Pseudomonas putida KT2440.
Indole-Induced Activities of β-Lactamase and Efflux Pump Confer Ampicillin Resistance in Pseudomonas putida KT2440.
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Indole induces RND efflux pump expression contributing to ampicillin resistance
"Among the genes involved in β-lactam resistance (Nakae et al., 1999; Quale et al., 2006; Kong et al., 2010), genes encoding resistance nodulation cell division (RND) efflux pumps/transporters (ttgABC, acrB2, and acrB3) and bacterial secretion systems, specifically, were increased by indole plus Amp or only indole (Supplementary Tables S5–S7), suggesting that they might contribute primarily to the acquisition of indole-induced Amp resistance"
Coordinate overexpression of two RND efflux systems, ParXY and TtgABC, is responsible for multidrug resistance in Pseudomonas putida.
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TtgR mutations leading to TtgABC overproduction cause increased resistance to fluoroquinolones and beta-lactams
"Mutants of the first type were more resistant to fluoroquinolones and beta-lactams except imipenem, and overproduced the efflux system TtgABC"
Resistance to Bipyridyls Mediated by the TtgABC Efflux System in Pseudomonas putida KT2440.
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ttgB deletion makes KT2440 susceptible to bipyridyl compounds and caerulomycin A
"the deletion of ttgB made the strain susceptible to 2,2'-bipyridyl and natural bipyridyl derivatives such as caerulomycin A, indicating that TtgABC is required for detoxification of compounds of the bipyridyl family"
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Addition of copper restores growth of ttgB mutant
"the addition of copper restores the growth of the ttgB mutant and the production of pyoverdine"
Deep research on ttgB in Pseudomonas putida KT2440
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TtgB is the inner-membrane RND transporter of the TtgABC tripartite efflux pump
"TtgA (PP_1386) is the periplasmic adaptor/MFP, TtgB (PP_1385; Q88N31) is the inner-membrane RND transporter/IMP, and TtgC (PP_1384) is the outer-membrane channel component"