Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids.
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beta3Gn-T5 (B3GNT5) is the Lc3Cer synthase; it transfers GlcNAc to lactosylceramide and to nLc4Cer, producing Lc3Cer and nLc5Cer respectively, the entry point for lacto/neolacto-series glycolipids carrying HNK-1 and Lewis X epitopes.
"beta3Gn-T5 exhibited strong activity to transfer GlcNAc to glycolipid substrates, such as lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively"
Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis.
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The mouse ortholog (Lc3 synthase) initiates lacto-series glycolipid synthesis using the lactosylceramide acceptor; it is developmentally regulated with elevated expression in the developing CNS.
"The Lc3 synthase enzyme efficiently utilized the lactosyl ceramide glycolipid acceptor"
A reference map of the human binary protein interactome.
Glycosphingolipid biosynthesis
B3GNT5 transfers GlcNAc to LacCer