Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The beta(2)-adrenergic receptor mediates extracellular signal-regulated kinase activation via assembly of a multi-receptor complex with the epidermal growth factor receptor.
G protein-coupled receptors form stable complexes with inwardly rectifying potassium channels and adenylyl cyclase.
Beta(1)/beta(2)/beta(3)-adrenoceptor knockout mice are obese and cold-sensitive but have normal lipolytic responses to fasting.
Ligand-regulated internalization and recycling of human beta 2-adrenergic receptors between the plasma membrane and endosomes containing transferrin receptors.
Hetero-oligomerization between beta2- and beta3-adrenergic receptors generates a beta-adrenergic signaling unit with distinct functional properties.
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Beta2 adrenergic receptor can homo- and hetero-oligomerize, stimulate adenylyl cyclase, recruit beta-arrestin, and alter G protein coupling in beta2/beta3 receptor signaling units.
Biochemical and biophysical characterization of serotonin 5-HT2C receptor homodimers on the plasma membrane of living cells.
A system for quantifying dynamic protein interactions defines a role for Herceptin in modulating ErbB2 interactions.
Dosage-dependent switch from G protein-coupled to G protein-independent signaling by a GPCR.
The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.
Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL.
Structure of an arrestin2-clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking.
Ligand-regulated oligomerization of beta(2)-adrenoceptors in a model lipid bilayer.
Beta-2 adrenergic receptor mediated ERK activation is regulated by interaction with MAGI-3.
Binding of amyloid beta peptide to beta2 adrenergic receptor induces PKA-dependent AMPA receptor hyperactivity.
Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to mediate ubiquitination of the beta2-adrenergic receptor.
Physical and functional interaction between CB1 cannabinoid receptors and beta2-adrenoceptors.
SNX27 mediates PDZ-directed sorting from endosomes to the plasma membrane.
MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors.
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MARCH2 promotes ligand-specific beta2AR ubiquitination, endocytosis, lysosomal sorting, and degradation.
Distinct roles for β-arrestin2 and arrestin-domain-containing proteins in β2 adrenergic receptor trafficking.
Mammalian α arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with β arrestins.
Adenosine A1 receptors heterodimerize with β1- and β2-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling.
Structural basis for endosomal trafficking of diverse transmembrane cargos by PX-FERM proteins.
β-adrenergic receptor-stimulated lipolysis requires the RAB7-mediated autolysosomal lipid degradation.
CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum.
Insights into β2-adrenergic receptor binding from structures of the N-terminal lobe of ARRDC3.
S-Palmitoylation of a Novel Site in the β2-Adrenergic Receptor Associated with a Novel Intracellular Itinerary.
Structure of the gene for human beta 2-adrenergic receptor: expression and promoter characterization.
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Site-directed mutagenesis and continuous expression of human beta-adrenergic receptors. Identification of a conserved aspartate residue involved in agonist binding and receptor activation.
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Human beta-2 adrenergic receptor mutagenesis supports catecholamine binding, Gs coupling, adenylate cyclase stimulation, and beta-adrenergic receptor activation.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Quantitative fragmentomics allow affinity mapping of interactomes.
Multiplexed mapping of the interactome of GPCRs with receptor activity-modifying proteins.
Amino-terminal polymorphisms of the human beta 2-adrenergic receptor impart distinct agonist-promoted regulatory properties.
A novel interaction between adrenergic receptors and the alpha-subunit of eukaryotic initiation factor 2B.
Role of clathrin-mediated endocytosis in agonist-induced down-regulation of the beta2-adrenergic receptor.
The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange.
A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins.
Beneath the surface: endosomal GPCR signaling.
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Some GPCRs, including beta2-adrenergic receptor, continue to activate G proteins after internalization into endosomes, and a single receptor can simultaneously engage G protein via its transmembrane core and beta-arrestin via its phosphorylated tail in a megaplex assembly, so endosomes can be a signaling-competent compartment rather than only a degradation/recycling route.
Update on the Role of beta2AR and TRPV1 in Respiratory Diseases.
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In respiratory tissues beta2AR couples to Gs/cAMP/PKA driving bronchodilation, mucociliary clearance, and anti-inflammatory effects, while GRK/beta-arrestin signaling drives desensitization and internalization and links to ERK, p38 MAPK, and NF-kB inflammatory transcriptional programs.
Liganded Gs-activating GPCR acts as a GEF for Gs
USP20, USP33 deubiquitinate ADRB2
The Ligand:GPCR:Gs complex dissociates
Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
ADRB2 in ADRB2:GRK complex is phosphorylated
ADRB2:Catecholamine binds ARRB1, ARRB2
ARBB recruits GPCRs into clathrin-coated pits
F- and N- BAR domain proteins bind the clathrin-coated pit
CLASP proteins and cargo are recruited to the nascent clathrin-coated pit
Clathrin recruits PIK3C2A
Clathrin-associated PIK3C2A phosphorylates PI(4)P to PI(3,4)P2
SNX9 recruits components of the actin polymerizing machinery
BAR domain proteins recruit dynamin
SYNJ hydrolyze PI(4,5)P2 to PI(4)P
Endophilins recruit synaptojanins to the clathrin-coated pit
HSPA8-mediated ATP hydrolysis promotes vesicle uncoating
Clathrin recruits auxilins to the clathrin-coated vesicle
Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle
Dynamin-mediated GTP hydrolysis promotes vesicle scission
Dissociation of clathrin-associated proteins
Dissociation of AAK1 and dephosphorylation of AP-2 mu2
RAB5 and GAPVD1 bind AP-2
ADRB2:GRK complex dissociates to Phosphorylated ADRB2
GRKs bind ADRB2:Catecholamine
β-blockers bind ADRB1,2,3
β1,2-agonists bind ADRB1,2
UniProtKB record for human ADRB2
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UniProt summarizes ADRB2 as a catecholamine GPCR that couples to Gs and Gi proteins, binds epinephrine with higher affinity than norepinephrine, localizes primarily to the cell membrane, and undergoes endosomal, lysosomal, and Golgi-associated trafficking.