Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation.
Characterization of a bifunctional archaeal acyl coenzyme A carboxylase.
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In the Acidianus brierleyi ortholog, the acyl-CoA and carboxybiotin binding sites are in the carboxyltransferase subunit.
"The acyl-CoA-binding site and the carboxybiotin-binding site were found in the carboxyltransferase subunit."
Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO<sub>2</sub> incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus.
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Recombinant alpha and gamma subunits made in E. coli were insoluble and inactive. Active recombinant ACC, His-tagged on the gamma subunit, was purified from P. furiosus co-expressing the M. sedula biotin protein ligase.
"Recombinant ACC purified from MW112 cell extract (Table II) was functional"
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide.
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Msed_1375 is E1-gamma of the acetyl/propionyl-CoA carboxylase expressed in P. furiosus.
"E1 (acetyl/propionyl-CoA carboxylase, encoded by Msed_0147, Msed_0148, and Msed_1375)"
Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.
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Msed_1375 is induced with the other ACC subunit genes under autotrophic growth.
"especially the genes encoding acetyl-CoA/propionyl-CoA carboxylase (ACCαβγ) (Msed_0147, Msed_0148, Msed_1375; 7- to 30-fold)"
UniProt record A4YGI1 (A4YGI1_METS5)