Gene Ontology annotation through association of InterPro records with GO terms.

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods.

PMID:22982048

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.

This paper supports a macroautophagy/lysosome context, but not a specific ATP6V0B regulatory function.

"macroautophagy is responsible for the uptake of lipofuscin into the lysosomes"

PMID:23864651

The identification of novel proteins that interact with the GLP-1 receptor and restrain its activity.

The GLP-1R interaction study used a membrane yeast two-hybrid screen and does not define ATP6V0B core molecular function.

"A screen of a human fetal brain cDNA prey library with an unliganded human GLP-1R as bait in yeast revealed 38 novel interactor protein candidates."

PMID:32001091

Structure and Roles of V-type ATPases.

V-ATPases are membrane-embedded ATP-driven proton pumps and are the primary source of organellar acidification in eukaryotes.

"V-ATPases are membrane-embedded protein complexes that function as ATP hydrolysis-driven proton pumps."

PMID:32296183

A reference map of the human binary protein interactome.

The HuRI study is a large-scale binary interactome resource, not ATP6V0B-specific functional evidence.

"The dataset, versioned HI-III-20 (Human Interactome obtained from screening Space III, published in 2020), contains 52,569 verified PPIs involving 8,275 proteins"

PMID:33065002

Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.

Human V-ATPase is an ATP-driven proton pump with a cytoplasmic V1 ATP-hydrolysis sector and a membrane-embedded Vo proton-transfer sector.

"Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer."
Organellar V-ATPases maintain endosome and lysosome pH homeostasis and support trafficking and protein degradation.

"Vesicular and organellar V-ATPases are essential in establishing and maintaining the pH homeostasis of endosomes and lysosomes and in supporting intracellular membrane trafficking and protein degradation"

PMID:9653649

Identification and characterization of the gene encoding a second proteolipid subunit of human vacuolar H(+)-ATPase (ATP6F).

ATP6V0B/ATP6F is the human second V-ATPase proteolipid, a five-transmembrane c subunit with conserved Glu98 required for H+ transport.

"hATP6F is a hydrophobic protein with five putative transmembrane segments, having 61% amino acid identity and 83% similarity to the yeast protein, except in the N-terminus, and contains a conserved glutamic acid residue (Glu98) that is essential for H(+)-transporting activity."

Reactome:R-HSA-1222516

Intraphagosomal pH is lowered to 5 by V-ATPase

Reactome describes V-ATPase rotary pumping into the phagosome.

"When pumping, ATP hydrolysis drives a 120 degree rotation of the rotor which leads to movement of three protons into the phagosome"

Reactome:R-HSA-5252133

ATP6AP1 binds V-ATPase

Reactome:R-HSA-74723

Endosome acidification

Reactome describes endosomal acidification as proton-pump driven entry of H+ into the endosome lumen.

"The effect of the proton pump is to allow entry of [H+] ions into the lumen of the endosome."

Reactome:R-HSA-917841

Acidification of Tf:TfR1 containing endosome

Reactome:R-HSA-9639286

RRAGC,D exchanges GTP for GDP

Reactome:R-HSA-9640167

RRAGA,B exchanges GDP for GTP

Reactome:R-HSA-9640168

v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP:SLC38A9:Arginine dissociates yielding v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP and SLC38A9:Arginine

Reactome:R-HSA-9640175

v-ATPase:Ragulator:RagA,B:GDP:RagC,D:GDP binds SLC38A9:Arginine

Reactome:R-HSA-9640195

RRAGA,B hydrolyzes GTP

Reactome:R-HSA-9645598

RRAGC,D hydrolyzes GTP

Reactome:R-HSA-9645608

v-ATPase:Ragulator:RRAGA,B:GTP:RRAGC,D:GDP binds mTORC1

Reactome places V-ATPase activity upstream of lysosomal mTORC1 recruitment.

"Hydrolysis of ATP by the v-ATPase complex is also required for recruitment of mTORC1"

Reactome:R-HSA-9646468

mTORC1 binds RHEB:GTP

Reactome:R-HSA-9858940

MITF-M-dependent ATP6V0B gene expression

Reactome records MITF-dependent expression of ATP6V0B and the lysosome/endosome acidification role of V-ATPase.

"MITF has been implicated in the regulation of expression of many components of the v-ATPase, including the transmembrane component ATP6V0B"

file:human/ATP6V0B/ATP6V0B-deep-research-falcon.md

Falcon deep research report on ATP6V0B

Falcon deep research supports ATP6V0B as the human V-ATPase V0 c'' proteolipid subunit and highlights the PN-relevant lysosomal acidification role.

"ATP6V0B-containing V-ATPases acidify lysosomes, endosomes, Golgi, and secretory/synaptic vesicles and can localize to plasma membranes in specialized cells."

file:human/ATP6V0B/ATP6V0B-deep-research-cyberian.md

Cyberian deep research on ATP6V0B function

file:projects/PROTEOSTASIS/reports/pn_projection/pn_projected_annotations.tsv

Proteostasis PN projected annotations for ATP6V0B

PN projection maps ATP6V0B in the V0 lysosomal V-ATPase proton pump component leaf to GO:0046610.

"ATP6V0B Autophagy-Lysosome Pathway|Pre-initiation autophagy signaling|mTORC1 pathway, upstream|Nutrient sensing|V0 lysosomal v-ATPase proton pump component"