Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:25190516

The small GTPase Arf1 modulates mitochondrial morphology and function

file:worm/miro-1/miro-1-deep-research-falcon.md

Deep research summary for miro-1 gene function

miro-1(tm1966) mutants have ~50% wild-type mitochondrial content

"miro-1(tm1966) mutants show ~50% of wild-type mitochondrial amount"
Reduced mitochondrial amount correlates with lifespan extension dependent on daf-16/FOXO
Oxygen consumption only mildly reduced despite reduced mitochondrial content
MIRO-1 physically interacts with VDAC-1

"MIRO-1 physically interacts with VDAC-1"
Required for maintaining mitochondrial membrane potential (measured by TMRE, JC-1)
EF-hand mutations impair membrane potential maintenance
MIRO-1 enriched on fragmented mitochondria during stress
MIRO-1/MTX-1/MTX-2/KLC-1 complex for kinesin-based anterograde transport

"MTX-1/2 bind MIRO-1 and kinesin light chain (KLC-1) to form adaptor complexes"
MIRO-1/MTX-2/TRAK-1 complex for dynein-based retrograde transport

"MTX-2, MIRO-1, TRAK-1 form a distinct adaptor for dynein-based transport"
Biochemical evidence from gel filtration and immunoprecipitation
Genetic evidence from PVD and DA9 neuron studies
MIRO-1 promotes RIC-7 recruitment to mitochondria

"MIRO-1 promotes recruitment/enrichment of RIC-7 on mitochondria"
MIRO-1 optimizes but is not strictly required for anterograde transport
miro-1 mutants show largely immobilized axonal mitochondria

"miro mutants show largely immobilized axonal mitochondria"
Neuron-specific effects on mitochondrial distribution
MIRO-1 required for Ca2+-dependent mitochondrial fragmentation after wounding

"Wounding triggers rapid, reversible mitochondrial fragmentation that requires MIRO-1 and cytosolic Ca2+"
Fragmentation accelerates actin-based wound repair
Links MIRO-1 calcium sensing to stress response
Comprehensive review of Miro domain architecture and function

"MIRO proteins have tandem GTPase domains, tandem EF-hand Ca2+-binding motifs, and regulate motor/adaptor interactions"
EF-hands as Ca2+ sensors regulate transport
PINK1/Parkin targeting of Miro during mitophagy
ER-mitochondria contact regulation