Vps52 (CG7371) notes

Shared core subunit of GARP (with Vps54/scat) and EARP (with Vps50/CG4996).

• Rab affinity chromatography in S2 cells: Vps51-53 are GTP-specific Rab4 interactors [PMID:25453831 "Most striking were Vps51, Vps52, and Vps53, three subunits of the GARP tethering complex that is found on the Golgi and involved in retrograde traffic from endosomes"; "we were able to confirm that GARP subunit binding is GTP specific"].
• CG4996 (Vps50) co-IPs Vps52 and Vps53: second GARP form, "GARPII" PMID:25453831. This is EARP PMID:25799061.
• Vps52-GFP is at the TGN in salivary gland and follicle cells; Arl5 aids recruitment [PMID:25795912 "Drosophila Vps52 localizes to the TGN."; "Removal of Arl5 reduced the intensity of Vps52-GFP on the Golgi and there was a substantial increase in the amount of GFP in the cytoplasm"].
• PMID:25795912 does not assay recycling to the plasma membrane, so its IDA "endocytic recycling" row was modified to retrograde transport, endosome to Golgi.
• EARP mediates transferrin recycling in human cells PMID:25799061.

Decisions: GARP complex ACCEPT; NEW EARP complex (IPI, PMID:25453831) for consistency with Vps51; contributes_to vesicle membrane tethering activity (GO:7770062) as core MF.

Deep research: falcon completed (genes/DROME/Vps52/Vps52-deep-research-falcon.md) after the review was first committed; checked for consistency.
- Deep research (falcon) agrees with the TGN localization and Arl5-dependent recruitment and adds no fly loss-of-function data for Vps52.