Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Characterization of human DNase I family endonucleases and activation of DNase gamma during apoptosis.
Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated deoxyribonuclease and the poly(ADP-ribose) polymerase-regulated DNAS1L3, an endoplasmic reticulum-localized endonuclease that translocates to the nucleus during apoptosis.
Host DNases prevent vascular occlusion by neutrophil extracellular traps.
A reference map of the human binary protein interactome.
Cloning and characterization of a novel human DNase.
Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I.
Cloning and characterization of an actin-resistant DNase I-like endonuclease secreted by macrophages.
Digestion of Chromatin in Apoptotic Cell Microparticles Prevents Autoimmunity.
DNaseY: a rat DNaseI-like gene coding for a constitutively expressed chromatin-bound endonuclease.
Involvement of DNase gamma in apoptosis associated with myogenic differentiation of C2C12 cells.
DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis.
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase.
cDNA cloning of human DNase gamma: chromosomal localization of its gene and enzymatic properties of recombinant protein.
DNASE1L3 maintains homeostatic levels of mitochondrial DNA on the mammalian cell surface.
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Mitochondrial DNA is tethered to the surface of select mammalian cells through cell surface proteins and heparan sulfate proteoglycans.
"we provide evidence that mitochondrial DNA is tethered to the surface of select mammalian cells through cell surface proteins and heparan sulfate proteoglycans"
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Secreted DNASE1L3 clears DNA from human and mouse B cell surfaces, and carriers of an autoimmunity-associated DNASE1L3 missense variant have more surface DNA on B and T cells.
"human and murine B cell surfaces contain DNA that can be cleared by the secreted nuclease DNASE1L3 and that patients with a DNASE1L3 missense variant associated with increased risk for autoimmune disease harbor increased levels of surface DNA on B and T cells"
DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells.
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Trypsin-released cell surface DNA is enriched for the mitochondrial genome and for 8-oxo-dG, and its surface presentation depends on heparan sulfate chains.
"Across all three cell lines tested, when comparing the whole cell DNA to the trypsin-release samples, we found significant enrichment across the mitochondrial genome"
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Among primary immune cells, surface DNA is found on B cells and monocytes.
"where it displays specificity for B cells and monocytes"
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Recombinant wild-type DNASE1L3, but not DNASE1, removes surface DNA from primary human B cells; its effect on monocytes varies by donor.
"DNASE1L3, but not DNASE1, partially and reproducibly removed cell surface DNA"
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Homozygotes for the secretion-defective R206C risk allele (rs35677470) have more surface DNA on B cells and detectable surface DNA on T cells.
"In individuals with this variant, we detected an increase in csATAC-See signal on B cells, as well as increased signal on T cells to levels above background"
Reduced digestion of circulating genomic DNA in systemic sclerosis patients with the DNASE1L3 R206C variant.
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The R206C risk variant impairs DNASE1L3 secretion, and plasma from carriers digests apoptotic membrane-vesicle DNA less well and carries longer circulating DNA fragments.
"Extracellular secretion of DNASE1L3 R206C was impaired."
Dnase1l3 deletion causes aberrations in length and end-motif frequencies in plasma DNA.
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Dnase1l3-/- mice have altered plasma DNA fragment length and end-motif frequencies, independent of their anti-DNA response.
"DNASE1L3 plays a role in circulating plasma DNA homeostasis by enhancing fragmentation and influencing end-motif frequencies"
Plasma DNA Profile Associated with DNASE1L3 Gene Mutations: Clinical Observations, Relationships to Nuclease Substrate Preference, and In Vivo Correction.
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Patients with DNASE1L3 variants show altered plasma DNA size and loss of the CC end motif; AAV-delivered Dnase1l3 restores end motifs in deficient mice.
"DNASE1L3 is an important player in the fragmentation of plasma DNA, which appears to act in a cell-extrinsic manner"
Autoantibody-mediated impairment of DNASE1L3 activity in sporadic systemic lupus erythematosus.
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Neutralizing autoantibodies reduce circulating DNASE1L3 activity in many sporadic SLE nephritis patients, with accumulation of microparticle-associated cfDNA.
">50% of sporadic SLE patients with nephritis manifested reduced DNASE1L3 activity in circulation, which was associated with neutralizing autoantibodies to DNASE1L3"
Loss-of-function variant in DNASE1L3 causes a familial form of systemic lupus erythematosus.
Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.
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In Dnase1l3-/- mice, anti-dsDNA responses arise through extrafollicular B cell differentiation that needs T cell help, pDCs, type I interferon and endosomal TLRs.
"anti-DNA responses in Dnase1l3-/- mice require CD40L-mediated T cell help"