DTD1 (A0A2R8YCT7): evidence and exact-input prediction review

A0A2R8YCT7 is shorter than the experimentally characterized DTD1 editing core and lacks the conserved Gly-cisPro motif. ProtNLM describes the full editing mechanism, but the selected sequence does not preserve the machinery needed for that inference.

Input identity and functional boundary

A0A2R8YCT7 and Q8TEA8 share HGNC:16219. The selected 127 residues align to reference 1–127 with 125 identities. Reference Gly139–Pro140 is outside the selected product. This is a sequence deletion, not a measured cis-to-trans conformational change or evidence of conversion into the DTD2/ATD paralog.

Biological evidence

the N-terminal
core of DUE-B is shown to display both D-aminoacyl-tRNA deacylase activity and
ATPase activity.

how it uses an invariant
'cross-subunit' Gly-cisPro dipeptide to capture the chiral centre of incoming
D-aminoacyl-tRNA.

Exact non-GO claims

The complete emitted record is preserved in DTD1-protnlm-source.json. Assessments below address the selected protein product; evidence on longer products is identified explicitly.

Protein name

D-tyrosyl-tRNA(Tyr) deacylase

NPI (CS 0) as an enzymatic name for A0A2R8YCT7: the short sequence lacks the reference Gly-cisPro motif needed for the conserved editing site. “DTD1-derived fragment” captures the supported identity without assigning deacylase activity. Sequence mapping; PMID:24302572(https://pubmed.ncbi.nlm.nih.gov/24302572/).

Function

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality

NPI (CS 0) for the composite assertion that this selected protein executes the complete DTD editing mechanism. The family mechanism, D-aminoacyl-tRNA hydrolysis and chiral rejection are real, but its conserved Gly-cisPro element is deleted here. Gly-tRNA(Ala) hydrolysis and an in vivo protection phenotype have not been demonstrated for this truncated product. This does not challenge the full-length human DTD1 activity measured in PMID:17264083 or infer an ATD-like alternative specificity. PMID:17264083(https://pubmed.ncbi.nlm.nih.gov/17264083/); PMID:24302572(https://pubmed.ncbi.nlm.nih.gov/24302572/).

No GO or EC term was emitted in this record; the name, function and location assessments above constitute its prediction review.

Family integration

PTHR10472:SF5 supports DTD1 ancestry. Functional transfer still requires the dimeric editing pocket: the benchmark target lacks a conserved motif present in the same-gene reference. No paralog switch or ancestral function change is asserted.

Evidence limits

PMID:17264083 is abstract-only in the cache; the DTD structural/mechanistic paper has full text. Activity of the exact 127-residue product was not measured in these sources. No core MF is assigned. Proteomics-database cross-references alone do not establish the short product’s activity.

Exact sequence mapping: DTD1-bioinformatics/RESULTS.md. Global alignments can place nonhomologous alternative tails opposite gaps or distant residues; only conserved segments and explicitly retained feature intervals support functional transfer.

Research integration

The genuine Falcon report supplies literature synthesis for DTD1. Its full-length enzyme mechanism is interpreted through the exact127-residue input and the mapped absence of the Gly-cisPro site; activity of the longer human editing core is not an assay of this short product.