Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods.
Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue.
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Multicopy dksA suppresses elastase and rhamnolipid production indirectly, by inhibiting rhlI transcription.
"dksA inhibits rhlI, rhlAB, and lasB transcription"
DksA represses ribosomal gene transcription in Pseudomonas aeruginosa by interacting with RNA polymerase on ribosomal promoters.
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DksA represses rRNA transcription, binds rRNA promoters with RNAP in vivo and co-purifies with RNAP.
"The binding to rRNA promoters specifically increases during stringent response, and correlates with the binding of RNAP to these regions."
Role of a Zn-independent DksA in Zn homeostasis and stringent response.
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The paralog DksA2 (PA5536) lacks the zinc finger, is Zur-repressed, and can replace DksA in vivo; in vitro assays use DksA2 with E. coli RNAP.
"DksA2 can functionally substitute for the canonical DksA in vivo in Escherichia coli and P. aeruginosa"
Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions.