Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Transcriptional regulation, nucleotide sequence, and localization of the promoter of the catBC operon in Pseudomonas putida.
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catB and catC encode cis,cis-muconate lactonizing enzyme I and muconolactone isomerase
"The catB and catC genes encode cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1) and muconolactone isomerase (EC 5.3.3.4), respectively."
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catBC enzymes are required for dissimilation of benzoate to beta-ketoadipate
"These enzymes are required for the dissimilation of benzoate to beta-ketoadipate by Pseudomonas putida..."
Cloning and complete nucleotide sequence determination of the catB gene encoding cis,cis-muconate lactonizing enzyme.
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catB encodes cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1)
"The enzyme, cis,cis-muconate lactonizing enzyme I (MLEI; EC 5.5.1.1), has been proposed to play a key role in the beta-ketoadipate pathway of benzoate degradation."
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purified MLEI has an approximately 40 kDa subunit and is a key beta-ketoadipate-pathway enzyme
"The subunit Mr was 40,000 which was in close agreement with the nucleotide sequence data."
Enzymes of the beta-ketoadipate pathway in Pseudomonas putida: kinetic and magnetic resonance studies of the cis,cis-muconate cycloisomerase catalyzed reaction.
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cis,cis-muconate cycloisomerase catalyzes reversible conversion of cis,cis-muconate and (+)-muconolactone
"Steady-state kinetic analysis of the divalent metal ion requiring cis,cis-muconate cycloisomerase catalyzed interconversion of cis,cis-muconate and (+)-muconolactone..."
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the enzyme is divalent-metal dependent and has a manganous-ion binding site
"The titration of enzyme freed of divalent metal ion with manganous ion... indicates a single binding site per subunit..."
Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants.
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wild-type P. putida muconate cycloisomerase accepts muconate and selected nonchlorinated analogs but not chlorinated muconates
"We show that they transform, in addition to cis,cis-muconate, 3-fluoro-, 2-methyl-, and 3-methyl-cis, cis-muconate with high specificity constants but not 2-fluoro-, 2-chloro-, 3-chloro-, or 2,4-dichloro-cis,cis-muconate."
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chloromuconate cycloisomerase specificity requires additional catalytic features not present in the wild-type muconate cycloisomerase
"These results show that the different catalytic characteristics of muconate and chloromuconate cycloisomerases are due to a number of features that can be changed independently of each other."
Generation of a catR deficient mutant of P. putida KT2440 that produces cis, cis-muconate from benzoate at high rate and yield.
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cat operon induction in KT2440 depends on CatR and is important for benzoate utilization
"Transcriptome analysis showed that the cat operon was not induced in P. putida KT2440-JD1 in the presence of 5mM benzoate, due to a point mutation in the highly conserved DNA binding domain of the transcriptional regulator (catR) of the cat operon."
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blocking cat operon induction causes cis,cis-muconate accumulation from benzoate
"The mutant was no longer able to grow using benzoate as a sole carbon source, but co-metabolized benzoate to cis, cis-muconate during growth on glucose, which accumulated in the growth medium."
Research report - catB function in Pseudomonas putida KT2440
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catB corresponds to PP_3715 in the catR-catBCA aromatic catabolism cluster
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CatB is best annotated as a cytosolic Mn(2+)-dependent cis,cis-muconate cycloisomerase
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CatB functions in the catechol ortho-cleavage branch of the beta-ketoadipate pathway
UniProt entry Q88GK6
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Gene name catB, locus tag PP_3715, EC 5.5.1.1
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UniProt records Mn(2+) as cofactor and aromatic compound metabolism as pathway context
PANTHER family metadata for PTHR48073