Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
COMPASS: a complex of proteins associated with a trithorax-related SET domain protein.
The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4.
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.
COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression.
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression.
The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation.
Proteome survey reveals modularity of the yeast cell machinery.
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
Structural characterization of Set1 RNA recognition motifs and their role in histone H3 lysine 4 methylation.
Different roles of histone H3 lysine 4 methylation in chromatin maintenance.
Effect of histone methyltransferase gene mutations on sporulation in S. cerevisiae.
Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS.
Cotranslational assembly of the yeast SET1C histone methyltransferase complex.
Defining the budding yeast chromatin-associated interactome.
Structural analysis of the core COMPASS family of histone H3K4 methylases from yeast to human.
Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes.
H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A.
Catalytic and functional roles of conserved amino acids in the SET domain of the S. cerevisiae lysine methyltransferase Set1.
Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.
Counteracting H3K4 methylation modulators Set1 and Jhd2 co-regulate chromatin dynamics and gene transcription.
The histone methyltransferases Set5 and Set1 have overlapping functions in gene silencing and telomere maintenance.
RNA Binding by Histone Methyltransferases Set1 and Set2.
Repression of Middle Sporulation Genes in Saccharomyces cerevisiae by the Sum1-Rfm1-Hst1 Complex Is Maintained by Set1 and H3K4 Methylation.
Binding to RNA regulates Set1 function.
The cellular economy of the Saccharomyces cerevisiae zinc proteome.
Genetic Interactions of Histone Modification Machinery Set1 and PAF1C with the Recombination Complex Rec114-Mer2-Mei4 in the Formation of Meiotic DNA Double-Strand Breaks.
The social and structural architecture of the yeast protein interactome.
Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres.
SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes.
Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions.
Set1 regulates telomere function via H3K4 methylation-dependent and -independent pathways and calibrates the abundance of telomere maintenance factors.
Deep research report on SET1
Falcon deep research report on Saccharomyces cerevisiae SET1
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SET1/YHR119W (UniProt P38827) is the sole H3K4-specific SET-domain histone
methyltransferase in budding yeast and the catalytic subunit of COMPASS,
depositing mono-, di-, and trimethylation on histone H3 lysine 4.
"Set1/COMPASS deposits methyl groups on **histone H3 lysine 4 (H3K4)** in three states: **mono- (me1), di- (me2), and tri-methylation (me3)**"
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Yeast COMPASS is an eight-subunit complex (Set1 plus seven accessory
subunits) with a WRAD-like catalytic core (Swd1, Swd3, Bre2, Sdc1).
"COMPASS is an eight-subunit complex consisting of Set1 plus seven accessory subunits (Swd3/Cps30, Swd1/Cps50, Bre2/Cps60, Sdc1/Cps25, Spp1/Cps40, Swd2/Cps35, Shg1/Cps15)"
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Set1/COMPASS is recruited co-transcriptionally to actively transcribed
genes via interaction with the phosphorylated CTD of RNA Pol II (Rpb1).
"Set1/COMPASS preferentially associates with **actively transcribed genes** by interacting with the **phosphorylated C-terminal domain (CTD)** of Rpb1 (RNA polymerase II largest subunit)"
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A defining regulatory principle is trans-histone crosstalk: H2B
monoubiquitination at K123 (installed by Rad6/Bre1) is required for normal
levels of H3K4 di- and tri-methylation by COMPASS.
"A defining regulatory principle for Set1/COMPASS is **trans-histone crosstalk** in which **H2B monoubiquitination at K123 (H2BK123ub)** (installed by Rad6/Bre1) is required for normal levels of **H3K4 di- and tri-methylation** by COMPASS"
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Set1 regulates telomere biology through both H3K4 methylation-dependent
and -independent pathways: subtelomeric repression tracks with H3K4
methylation, whereas telomere length maintenance requires the Set1
catalytic core but is not strictly explained by H3K4 methylation status.
"Telomere length maintenance appears to require the Set1 catalytic core but is not strictly explained by H3K4 methylation status alone, implying a partially H3K4-substrate–independent catalytic role or additional substrates"
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N-terminal acetylation of COMPASS subunits fine-tunes H3K4 methylation:
NatA acetylates Shg1, Spp1, and Swd2, and NatA deletion decreases global
H3K4me3 and shifts H3K4me2 localization toward promoters (Woo et al. 2024).
"NatA is required for N-terminal acetylation of Shg1, Spp1, and Swd2, and NatA deletion decreases global H3K4me3 and shifts H3K4me2 localization toward promoters"
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Swd2/Cps35 is shared between COMPASS and the 3'-end CPF complex; Rad6
catalytic activity is essential for Swd2 chromatin binding, and Set1 helps
redistribute a limited Swd2 pool toward 5' regions for genome-wide H3K4me3
(Oh et al. 2024).
"They report that **Rad6 catalytic activity** is essential for Swd2 chromatin binding and that Set1 helps redistribute a limited Swd2 pool toward 5′ regions to achieve genome-wide H3K4me3"
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Beyond transcription, Set1-dependent H3K4me3 accumulates near DNA
double-strand breaks, and H3K4me2/3 are enriched at replication origins,
contributing to efficient DNA replication.
"H3K4me2/3 deposited by Set1 are enriched at **origins of replication** and contribute to efficient DNA replication, extending Set1 function beyond transcription into DNA replication-associated chromatin control"