Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2M2E3 (A0A0H2M2E3_VARPD) - Gram-negative bacterial tonB protein from Variovorax paradoxus TBEA6
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VPARA_25820 is a 232-aa inner-membrane single-pass TonB protein containing an N-terminal transmembrane helix (residues 18-35), a disordered periplasmic linker region (residues 58-143) with proline-rich segments (residues 59-87 and 95-135), and a C-terminal TonB domain (PROSITE PS52015, Pfam PF03544, residues 140-232).
"SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|ARBA:ARBA00004383, ECO:0000256|PROSITE-ProRule:PRU01359}; Single-pass membrane protein"
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VPARA_25820 corresponds to EMBL CDS accession KLN56276.1 on genomic contig JZWI01000012, where consecutive CDS accessions (KLN56276.1-KLN56277.1) and locus tags stepping by 10 place tonB (VPARA_25820) immediately upstream of bfrD1 (VPARA_25830, KLN56277.1).
"DR EMBL; JZWI01000012; KLN56276.1; -; Genomic_DNA."
TonB-dependent transporters: regulation, structure, and function.
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The inner-membrane TonB-ExbB-ExbD complex harvests the proton-motive force and interacts with the periplasmic side of outer-membrane TonB-dependent transporters to drive active import of ferric-siderophore complexes.
"The transport process requires energy in the form of proton motive force and a complex of three inner membrane proteins, TonB-ExbB-ExbD, to transduce this energy to the outer membrane."