Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The RdeA-RegA system, a eukaryotic phospho-relay controlling cAMP breakdown.
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RdeA is a phosphotransfer protein that supplies phosphate to RegA, the response-regulator cAMP phosphodiesterase.
"a phospho-transfer protein that supplies phosphates to RegA"
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Phosphotransfer is bidirectional and depends on His-65 of RdeA and Asp-212 of RegA.
"and aspartate 212 of RegA, and occurs in both directions"
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
Differentiation-inducing factor 2 modulates chemotaxis via the histidine kinase DhkC-dependent pathway in Dictyostelium discoideum.
A new class of rapidly developing mutants in Dictyostelium discoideum: implications for cyclic AMP metabolism and cell differentiation.
Evidence that the RdeA protein is a component of a multistep phosphorelay modulating rate of development in Dictyostelium.
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rdeA-null cells develop precociously, forming spores and stalk cells in ~14 h instead of ~24 h.
"aggregated rapidly and formed spores and stalk cells within 14 h of development instead of the normal 24 h"
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RdeA is a non-compartmentalised (cytosolic) ~28 kDa protein with a phosphoaccepting histidine at position 65, homologous to H2-module phosphotransferases.
"RdeA displays homology around a histidine residue at amino acid 65 with members of the H2 module family of phosphotransferases that participate in multistep phosphoryl relays"
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RdeA is functionally interchangeable with the yeast H2-module protein Ypd1.
"complemented by transformation with the Ypd1 gene of Saccharomyces cerevisiae, itself an H2 module protein"