Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Electron paramagnetic resonance study reveals a putative iron-sulfur cluster in human rpS3 protein.
Characterization of human ribosomal protein S3 binding to 7,8-dihydro-8-oxoguanine and abasic sites by surface plasmon resonance.
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RPS3/hS3 binds damaged DNA lesions, including 8-oxoG and AP DNA, with high affinity.
"An even more dramatic five orders of magnitude higher binding affinity for AP DNA was found for hS3 as opposed to hOGG1."
RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis.
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RPS3 overexpression can activate caspase-dependent apoptosis in a cytokine-treatment context.
"Transient expression of GFP-rpS3 activates caspase-8/caspase-3 and sensitizes cytokine-induced apoptosis."
Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding proteins.
Human ribosomal protein S3 interacts with DNA base excision repair proteins hAPE/Ref-1 and hOGG1.
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Human RPS3/hS3 interacts with OGG1 and APE/Ref-1 and may influence DNA repair activities at damage sites.
"These results raise the possibility that hS3 not only functions as a ribosomal protein but, in addition, may influence repair activities at sites of DNA damage."
Characterization of a wide range base-damage-endonuclease activity of mammalian rpS3.
Mass spectrometric analysis of the human 40S ribosomal subunit: native and HCV IRES-bound complexes.
Erk phosphorylates threonine 42 residue of ribosomal protein S3.
The high binding affinity of human ribosomal protein S3 to 7,8-dihydro-8-oxoguanine is abrogated by a single amino acid change.
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK.
Knockdown of ribosomal protein S3 protects human cells from genotoxic stress.
Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein.
Molecular composition of IMP1 ribonucleoprotein granules.
Translocation of human ribosomal protein S3 to sites of DNA damage is dependant on ERK-mediated phosphorylation following genotoxic stress.
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation.
Ribosomal proteins are targets for the NEDD8 pathway.
Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.
[Interactions of human ribosomal protein S3 with undamaged and damaged DNA].
Human ribosomal protein S3 (hRpS3) interacts with uracil-DNA glycosylase (hUNG) and stimulates its glycosylase activity.
PKCdelta-dependent functional switch of rpS3 between translation and DNA repair.
Arginine methylation of ribosomal protein S3 affects ribosome assembly.
Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain.
Defining the membrane proteome of NK cells.
Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function.
RpS3 translation is repressed by interaction with its own mRNA.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Ribosomal protein S3, a new substrate of Akt, serves as a signal mediator between neuronal apoptosis and DNA repair.
Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis.
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40.
Characterization of the proteome, diseases and evolution of the human postsynaptic density.
IKKβ phosphorylation regulates RPS3 nuclear translocation and NF-κB function during infection with Escherichia coli strain O157:H7.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase.
Ribosomal protein S3 is stabilized by sumoylation.
A bead-based approach for large-scale identification of in vitro kinase substrates.
Hydrogen sulfide-linked sulfhydration of NF-κB mediates its antiapoptotic actions.
Ribosomal protein S3 interacts with TRADD to induce apoptosis through caspase dependent JNK activation.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Ribosomal protein S3 localizes on the mitotic spindle and functions as a microtubule associated protein in mitosis.
Structures of the human and Drosophila 80S ribosome.
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Human 80S ribosome structures support RPS3's core role as a structural constituent of the cytosolic ribosome/small ribosomal subunit.
"Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps."
Cytoplasmic ribosomal protein S3 (rpS3) plays a pivotal role in mitochondrial DNA damage surveillance.
AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation.
Ribosomal protein S3 interacts with the NF-κB inhibitor IκBα.
Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease.
Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Structure of the human 80S ribosome.
Caspase-3 cleaved p65 fragment dampens NF-κB-mediated anti-apoptotic transcription by interfering with the p65/RPS3 interaction.
Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.
ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Parkinson's disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Systematic identification of post-transcriptional regulatory modules.
Multimodal cell maps as a foundation for structural and functional genomics.
Implication of mammalian ribosomal protein S3 in the processing of DNA damage.
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Historical biochemical evidence connects mammalian ribosomal protein S3 with AP/UV endonuclease activities, but this is secondary to the core ribosomal role.
"The nuclease activities have been purified from mouse cells to yield a peptide of M(r) = 32,000, whose sequence indicates identity with ribosomal protein S3."
Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.
Falcon deep research on RPS3 GO-relevant functions
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RPS3's core GO annotations should prioritize ribosome structure, cytosolic small ribosomal subunit localization, and translation.
"RPS3 is a core protein of the **cytosolic small ribosomal subunit (40S)** and therefore supports GO annotations centered on (i) **structural constituent of ribosome**, (ii) **cytoplasmic translation**, and (iii) localization to **cytosolic ribosome / small ribosomal subunit / cytosol (cytoplasm)**."
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DNA repair and NF-kB/apoptosis annotations are secondary moonlighting contexts that require cautious evidence handling.
"A second tier of GO-relevant “extra-ribosomal” evidence (DNA lesion binding/BER cofactor activity, NF-κB transcriptional specificity, apoptosis) exists, but requires careful caveats about direct catalytic activity vs **cofactor/interaction** roles, species extrapolations, and the frequent reliance on **review synthesis** rather than modern physiological genetics."
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Mitochondrial inner membrane and ER localization should not be retained without direct RPS3-specific localization evidence.
"**Mitochondrial inner membrane / ER:** no direct, RPS3-specific evidence in the retrieved corpus supports stable mitochondrial inner membrane or ER localization; avoid those CC annotations without targeted localization data."
Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation
Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA
Formation of translation initiation complexes containing mRNA that does not circularize
Nascent polypeptide:mRNA:ribosome complex binds signal recognition particle (SRP)
Synthesis of nascent polypeptide containing signal sequence
18SE pre-rRNA in pre-40S particles is nucleolytically processed during translocation from the nucleus to the cytosol
eIF2:GTP is hydrolyzed, eIFs are released
eIF5B:GTP is hydrolyzed and released
The 60S subunit joins the translation initiation complex
Release of 40S and 60S subunits from the 80S ribosome
eIF3 and eIF1A bind to the 40S subunit
Formation of the 43S pre-initiation complex
Formation of UPF1:eRF3 complex on mRNA with a premature termination codon and no Exon Junction Complex
p-4S-UPF1 recruits SMG5, SMG7, SMG6, PNRC2, DCP1A, and PP2A
UPF1 binds an mRNP with a termination codon preceding an Exon Junction Complex
SMG6 hydrolyzes mRNA with premature termination codon
SMG1 phosphorylates UPF1 (enhanced by Exon Junction Complex)
SARS-CoV-1 nsp1 binds to 40S ribosomal subunit
SARS-CoV-2 nsp1 binds to 40S ribosomal complex
RQT complex binds K63polyUb-80S ribosome:no-go mRNA:peptidyl-tRNA with nascent peptide
RQT complex:K63polyUb-80S ribosome dissociates yielding K63polyUb-40S subunit and 60S subunit:peptidyl-tRNA with nascent peptide
ZNF598 K63 polyubiquitinates RPS10 (eS10) and RPS20 (uS10) of the 40S ribosomal subunit
ZNF598 and Ub:UBE2D1,2,3 bind stalled 80S ribosome
PELO:HBS1L-1:GTP binds 80S ribosome:non-stop mRNA:peptidyl-tRNA with nascent peptide
ABCE1:ATP binds PELO:HBS1L-1:GTP:80S ribosome:non-stop mRNA:peptidyl-tRNA with nascent peptide and HBS1L-1:GDP is released
ABCE1:PELO:80S Ribosome:non-stop mRNA:peptidyl-tRNA with elongating peptide dissociates yielding ABCE1:40S ribosomal subunit, PELO, and 60S ribosomal subunit:peptidyl-tRNA with nascent peptide