Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UniProtKB Q9C1W3 (ERG1_SCHPO) record
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
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Fission-yeast erg1 is anaerobically induced independently of Sre1p, unlike later oxygen-consuming ergosterol-pathway genes.
"Importantly, erg1 + , the first oxygen-requiring step in the pathway, was induced anaerobically but was Sre1p independent."
A genetic and pharmacological analysis of isoprenoid pathway by LC-MS/MS in fission yeast.
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LC-MS/MS of fission-yeast isoprenoid intermediates showed the expected accumulation of squalene and decrease in lanosterol after terbinafine inhibition of Erg1.
"terbinafine significantly decreased lanosterol level and increased squalene level in wild-type cells"
Systematic Target Screening Revealed That Tif302 Could Be an Off-Target of the Antifungal Terbinafine in Fission Yeast.
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Terbinafine inhibits fission-yeast Erg1, leading to intracellular squalene accumulation.
"Moreover, inhibition of Erg1 leads to the intracellular accumulation of squalene, a toxic metabolite that leads to rapid cell death"