title: DPM1 sperm-nuclear source record
gene: DPM1
This is a numerical extract and source locator for an existing measurement, not an independent experiment or a claim of nuclear catalytic activity.
Source: Judit Castillo Corullón (2014), Caracterització del contingut proteic del nucli de l’espermatozoïde humà i el seu potencial epigenètic, doctoral thesis, Universitat de Barcelona. Institutional catalogue; persistent identifier 2445/60858. The catalogue issue date is 2014-09-19; the PDF prints July 2014. Original institutional PDF. Annex 8.2, printed page 191, identifies de Mateo et al. (2011), Proteomic characterization of the human sperm nucleus, Proteomics 11:2714–2726 (PMID:21630459(https://pubmed.ncbi.nlm.nih.gov/21630459/)), and its supplementary information. The thesis supplies the target table missing from the abstract-only normal publication cache.
The institutional item record identifies the title and persistent handle. Its ORIGINAL bundle contains JCC_TESI.pdf, bitstream 748602ae-3122-4398-88b1-5043df608fcb. The catalogue lists 3,723,500 bytes and MD5 f0340b61f5775f4ae3f31ee52cc254e1; both match the saved original PDF. The HTML title, HTML citation metadata and API title agree exactly. This mapping registers the catalogue citation while retaining the original PDF as the measurement source.
PDF SHA-256: a3928145aa1e94f82f272a8f37befeb3d701582fc9fe9cb10f885438de5fc5a6. The source PDF is retained at the institutional repository; it is not reproduced here. The text-derived record was checked against the saved authentic PDF’s extraction. Source figure pixels and DPM1 peptide spectra were not independently inspected.
Table 8.1, printed page 193 / physical PDF page 194, lists proteins identified from isolated normozoospermic human sperm nuclei. The headings distinguish gene, protein, UniProt accession, sequence coverage and peptide count.
| Gene | UniProt accession | Sequence coverage (%) | Peptides |
|---|---|---|---|
| DPM1 | O60762 | 13.1 | 2 |
The reported protein is dolichol-phosphate mannosyltransferase. These values are the source’s target-identification fields, not independently recalculated MS results. No peptide sequences or spectrum-level assignment are inferred.
The original Catalan Methods are paraphrased here; no new verbatim source passage is reproduced. Printed pages 97–100 describe CTAB/digitonin treatment and repeated washing of the nuclear preparation, with microscopy assessment. The procedure-efficiency estimate of approximately 99% is distinct from the 2011 article abstract’s reported 99.9% nuclear purity; these are not interchangeable measurements. Printed pages 112–113 describe tryptic LC-MS/MS, the human Swiss-Prot search, FDR 0.05 and a minimum of two peptides including at least one unique peptide. The figure caption describes a blank-gel-band control.
The target table provides positive detection in the stated preparation. It does not establish permanent nuclear residence, an import mechanism or a nuclear catalytic role for DPM1. Microscopy of the preparation is not DPM1-specific immunolocalization. The evidence therefore supports a contextual, non-core localization assessment with these limits.