Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Human sphingosine-1-phosphate lyase: cDNA cloning, functional expression studies and mapping to chromosome 10q22(1).
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Original cloning and functional expression of human SGPL1; establishes it as the enzyme catalyzing the last, irreversible step of sphingolipid breakdown and identifies Cys-218/Cys-317 as important for catalysis.
"Sphingosine-1-phosphate lyase catalyzes the last step in sphingolipid breakdown, the cleavage of phosphorylated sphingoid bases such as sphingenine-1-phosphate."
Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.
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Recombinant human SGPL1 is enzymatically active, localizes to the ER, lowers sphingosine/S1P, and (lyase-activity-dependently) potentiates stress-induced ceramide accumulation and apoptosis.
"sphingosine-1-phosphate lyase overexpression in HEK293 cells decreases sphingosine and sphingosine 1-phosphate amounts but elevates stress-induced ceramide generation and apoptosis."
Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
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Large-scale innate-immunity interactome (HI5) that includes SGPL1 among the network proteins; notes SGPL1 regulates cell apoptosis. IntAct records the SGPL1-STING1 (Q86WV6) binary interaction.
"Similarly, BAT3 and SGPL1 also regulate cell apoptosis"
First evidence of sphingosine 1-phosphate lyase protein expression and activity downregulation in human neoplasm: implication for resistance to therapeutics in prostate cancer.
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Measures SPL protein expression and enzymatic activity in human prostate tissue; SPL drives irreversible S1P degradation and is lost in tumors, inversely correlated with SphK1.
"This enzyme drives irreversible degradation of sphingosine 1-phosphate (S1P)"
Orally active 7-substituted (4-benzylphthalazin-1-yl)-2-methylpiperazin-1-yl]nicotinonitriles as active-site inhibitors of sphingosine 1-phosphate lyase for the treatment of multiple sclerosis.
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Discovery of active-site S1P-lyase inhibitors with a co-crystal structure of the homodimeric human enzyme bound to PLP and inhibitor; oral dosing reduces peripheral T-cell numbers and protects in a rat MS model.
"as seen in the cocrystal structure of derivative 31 with the homodimeric human S1P lyase."
Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency.
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Biallelic SGPL1 mutations cause a syndromic steroid-resistant nephrotic syndrome with ichthyosis, adrenal insufficiency, immunodeficiency, and neurological defects; all mutations reduce/abolish SGPL1 protein and/or activity, and WT (not variant) human SGPL1 rescues S1P-lyase-deficient yeast.
"expression of WT human SGPL1 rescued growth of SGPL1-deficient dpl1Δ yeast strains, whereas expression of disease-associated variants did not."
A reference map of the human binary protein interactome.
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HuRI all-by-all binary interactome; source of the high-throughput SGPL1 "protein binding" hits (many ER/membrane partners). No specific physiological complex established.
"Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'."
PXLP-SGPL1 cleaves sphingoid-1-phosphates