PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily.
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Purified PA0305 degrades AHLs with C6 to C14 acyl chains.
"Using a biosensor assay, PA0305his was shown to degrade AHLs with acyl side chains ranging in length from 6 to 14 carbons."
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The protein is processed into alpha and beta subunits with the catalytic serine at the beta N-terminus.
"PA0305 undergoes post-translational processing resulting in α- and β-subunits, with the catalytic serine as the first amino acid of the β-subunit"
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The authors conclude it is an efficient long-chain AHL acylase.
"the pa0305 gene encodes an efficient acylase with activity towards long-chain homoserine lactones"
Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1.
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A PA0305 mutant grows normally on decanoyl-HSL; QuiP, not PA0305, is the acylase for AHL-dependent growth.
"Moreover, it appears that gene PA1032, and not the other genes encoding the other three Ntn hydrolase homologs, likely encodes the acyl-HSL acylase activity observed during the growth utilization of acyl-HSLs."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt