Full text of PMID:22609922 cached and read.
- PMID:22609922; coupled TrxA/TrxB/NADPH assay with no activity when either is omitted; Km(app) for H2O2, CHP and BHP measured.
- PMID:22609922, complemented in trans; not sensitive at 20 mM.
- PhoA/LacZ fusions: probably cytoplasmic, unlike E. coli Tpx PMID:22609922.
- IscR represses tpx; induced by redox-cycling agents and diamide.
- GO:0008379 thioredoxin peroxidase activity is labelled obsolete in current GOA; replaced here by GO:0140824 thioredoxin-dependent peroxiredoxin activity (substrate breadth shown), following the precedent in genes/XANCP/Q8P365.
| PTN node | node term | n_seeds | leaf IBAs | seed annotation (term, evidence, PMID) | verdict | rationale | other seeds | IBD stands without this seed? |
|---|---|---|---|---|---|---|---|---|
| PTN002454730 | GO:0008379 thioredoxin peroxidase activity | 2 | 32 (30 taxa) | GO:0008379, IDA, PMID:22609922 | SOUND | Purified enzyme assayed with Trx/TrxR/NADPH and three peroxides. | E. coli Tpx P0A862 (GO:0008379 IDA PMID:7499381; GO:0140824 EXP PMID:12514184) | Yes |
| PTN002454730 | GO:0034599 cellular response to oxidative stress | 2 | 32 (30 taxa) | GO:0070301 cellular response to hydrogen peroxide, IMP, PMID:22609922 | SOUND | Deletion mutant H2O2 hypersensitivity with complementation, explained by the gene's own peroxidase activity. | E. coli Tpx P0A862 (GO:0034599 IMP PMID:8824604) | Yes |
No PseudoCAP defects. One GO_CENTRAL defect (OUTDATED_STATEMENT, CONFIRMED): the PTN002454730 IBD uses the now-obsolete GO:0008379; repoint to GO:0140824. The IBD is otherwise sound.
ACCEPT 3, MODIFY 3, TOO_GENERAL 5.