Gene Ontology annotation based on UniPathway vocabulary mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt:Q88NC9

UniProtKB/Swiss-Prot reviewed entry for ALGG_PSEPK (Q88NC9)

Reviewed entry identifies AlgG as mannuronan C5-epimerase (EC 5.1.3.37)
Signal peptide residues 1-25 and mature chain residues 26-519 support periplasmic localization
UniProt places AlgG in alginate biosynthesis and notes protection of nascent alginate from AlgL

PMID:12534463

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

Establishes PP_1283 as the KT2440 algG locus in the genome sequence

PMID:12775688

The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation

algG deletion caused AlgL-dependent degradation products instead of normal polymer
Catalytically inactive AlgG could still support polymer formation, implying a structural role in the biosynthetic complex

PMID:24398681

Catalytic Mechanism and Mode of Action of the Periplasmic Alginate Epimerase AlgG

Structural and mutational analysis identified His319 and Arg345 as key catalytic residues
AlgG is a processive periplasmic polymer-level epimerase with an electropositive substrate-binding groove

file:PSEPK/algG/algG-deep-research-falcon.md

Falcon deep research fallback report for algG

KT2440 assignment is supported by strong orthology and conserved gene-cluster context
Periplasmic localization and alginate-biosynthetic role are the strongest defensible GO claims for KT2440