Gene Ontology annotation reference GO_REF:0000002
Gene Ontology annotation reference GO_REF:0000003
Gene Ontology annotation reference GO_REF:0000117
The single NTPase gene of Drosophila melanogaster encodes an intracellular nucleoside triphosphate diphosphohydrolase 6 (NTPDase6).
-
The cloned fly enzyme hydrolyses UDP, GDP and IDP about equally well; other NDPs and NTPs are poor substrates.
"The enzyme hydrolyzed UDP, GDP, and IDP equally well whereas other NDP and NTP were poor substrates."
-
Activity is latent in intact transfected S2 cells and the protein is mainly in the ER.
"Fluorescence microscopy revealed that the protein was located primarily in the ER."
-
Disulfide-linked dimer formation, which inhibits activity, depends on C42 (transmembrane) and C447 (exoplasmic); these positions match O76268 (NTPase-PA) numbering.
"dimer formation required C42 in the transmembrane domain and C447 in the exoplasmic domain"
ire-1-dependent transcriptional up-regulation of a lumenal uridine diphosphatase from Caenorhabditis elegans.
-
UDA-1, the C. elegans member of the same PANTHER subfamily (SF127), is a UDP/GDPase with a luminal active site in a secretory-pathway compartment.
"UDA-1, is a UDP/GDPase encoded by the gene uda-1"
NTPase (O76268) re-review isoform identity and subfamily relatives
-
O76268 (PA) is a full-length isoform. PE and PF differ at the N-terminus or by an internal insertion, and the 271-residue PC/PD product lacks the transmembrane segment and ACR1.
"O76268 is a full-length isoform, not a fragment."