GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:15962010

Sulphatase activities are regulated by the interaction of sulphatase-modifying factor 1 with SUMF2.

The formylglycine (FGly) modification of an active-site cysteine, generated by SUMF1 within the ER, is required for the catalytic activity of the sulphatases (including GALNS); SUMF2 modulates SUMF1 activity.

PMID:18285341

Distinct effects of N-acetylgalactosamine-4-sulfatase and galactose-6-sulfatase expression on chondroitin sulfates.

Galactose-6-sulfatase (GALNS) hydrolyses sulfate groups of chondroitin sulfate; modulating GALNS expression (overexpression vs siRNA silencing) inversely changes cellular chondroitin sulfate content, demonstrating a role in chondroitin sulfate catabolism.

PMID:22940367

The structure of human GALNS reveals the molecular basis for mucopolysaccharidosis IV A.

Crystal structure of human GALNS (EC 3.1.6.4); the enzyme removes 6-sulfate groups from terminal N-acetylgalactosamine-6-sulfate (chondroitin-6-sulfate) and galactose-6-sulfate (keratan sulfate). Formylglycine-generating enzyme converts Cys79 to a formylglycine nucleophile; the enzyme is a Ca2+-binding homodimer.

PMID:23533145

In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.

Shotgun proteomics of exosomes from expressed prostatic secretions in urine detected ~900 proteins, among them GALNS, consistent with its presence in the secreted/extracellular-exosome fraction.

PMID:30760748

Expression, activity and localization of lysosomal sulfatases in Chronic Obstructive Pulmonary Disease.

Immunostaining of cultured lung fibroblasts shows GALNS in a lysosomal localisation in both ever smokers and COPD patients, confirming lysosomal localisation.

PMID:8325655

Mucopolysaccharidosis IV A: assignment of the human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) gene to chromosome 16q24.

Chromosome-mapping study assigning the human N-acetylgalactosamine-6-sulfate sulfatase (GALNS; EC 3.1.6.4) gene to chromosome 16q24; the paper does not assay a 4-sulfatase activity.

PMID:820716

N-acetylgalactosamine-6-sulfate sulfatase in man. Absence of the enzyme in Morquio disease.

Original demonstration of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) activity in man and its absence in Morquio disease; source of the EC 3.1.6.4 catalytic-activity assignment in UniProt.

Reactome:R-HSA-1630304

GALNS oligomer hydrolyses sulfate from Gal6S in keratan sulfate

Reactome:R-HSA-2263490

Defective GALNS does not hydrolyse sulfate from Gal6S in keratan sulfate

Reactome:R-HSA-6798751

Exocytosis of azurophil granule lumen proteins

file:human/GALNS/GALNS-uniprot.txt

UniProtKB P34059 (GALNS_HUMAN) curated record

FUNCTION: lysosomal enzyme that hydrolyzes sulfate groups from the glycosaminoglycans keratan sulfate and chondroitin-6-sulfate; SUBCELLULAR LOCATION lysosome; homodimer; formylglycine (3-oxoalanine) modification of Cys79 critical for activity; deficiency causes MPS4A (Morquio A).

"Lysosomal enzyme that hydrolyzes sulfate groups from CC glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6- CC sulfate (C6S)."

Reactome:R-HSA-2022857

Keratan sulfate degradation

Reactome:R-HSA-2206290

MPS IV - Morquio syndrome A

PMID:1522213

Mucopolysaccharidosis type IVA. N-acetylgalactosamine-6-sulfate sulfatase exonic point mutations in classical Morquio and mild cases.

PMID:24726177

Molecular testing of 163 patients with Morquio A (Mucopolysaccharidosis IVA) identifies 39 novel GALNS mutations.