GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Curated ortholog transfer supplies the Xenopus-supported DynAP claim.

GO_REF:0000033

Annotation inferences using phylogenetic trees

Four literal PAINT IBDs occur on the reconstructed human target lineage.

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

The UniProt DynAP vocabulary mapping agrees with the experimentally grounded compartment.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

The current target HPA record reports an approved cytosolic ICC/IF pool.

GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

The matching Kintoun rule captures the conserved motility consequence.

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

The fluid-movement-to-extracellular-location inference is inappropriate for this cytoplasmic assembly factor.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Matching Kintoun family, ortholog and rule evidence supports cytoplasmic assembly.

PMID:19052621

Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.

Human KTU deficiency and complementary model-organism experiments establish cytoplasmic outer- and inner-arm preassembly, including defective heavy-chain incorporation in Chlamydomonas.

PMID:23872636

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

DYX1C1 associates with DNAAF2 in targeted yeast two-hybrid and tagged cellular co-immunoprecipitation experiments.

PMID:27173435

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.

Affinity purification of the human organelle proteome supports a DNAAF2–UBE3D association.

PMID:28041644

Mutations in PIH1D3 Cause X-Linked Primary Ciliary Dyskinesia with Outer and Inner Dynein Arm Defects.

Human PIH1D3 and DNAAF2 interact in targeted yeast two-hybrid and reciprocal cellular co-immunoprecipitation assays.

PMID:29727692

C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.

C11ORF70 deficiency disrupts dynein arms, and ortholog experiments examine its intraflagellar-transport-dependent behavior.

PMID:29844425

The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones.

DNAAF2 associates strongly with DYX1C1 isoform a, while the proposed R2SD assembly remains hypothetical.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

The BioPlex networks contain UBE3D–DNAAF2 associations in human cell contexts.

PMID:40205054

Multimodal cell maps as a foundation for structural and functional genomics.

The primary U2OS AP-MS network contains a UBE3D-bait/DNAAF2-prey association.

PMID:29727693

Mutations in C11orf70 Cause Primary Ciliary Dyskinesia with Randomization of Left/Right Body Asymmetry Due to Defects of Outer and Inner Dynein Arms.

C11ORF70/CFAP300 associates with DNAAF2 in yeast two-hybrid and tagged co-immunoprecipitation experiments.

PMID:30561330

A liquid-like organelle at the root of motile ciliopathy.

Tagged Xenopus Ktu localizes to dynamic dynein axonemal particles with dynein subunits and other assembly factors.

PMID:33263282

Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins.

Xenopus Dnaaf2 occupies DynAPs whose dynein clients show compartmentalized behavior.

PMID:23525783

The establishment of rotational polarity in the airway and ependymal cilia: analysis with a novel cilium motility mutant mouse.

Mouse Ktu deficiency disrupts tracheal and ependymal dynein-dependent motility.

PMID:29741156

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly.

Zebrafish Ktu contributes to specific axonemal dynein assemblies with tissue-dependent compensation by Pih1d2.

PMID:22368283

Human PIH1 associates with histone H4 to mediate the glucose-dependent enhancement of pre-rRNA synthesis.

Human PIH1D1 promotes pre-rRNA transcription through histone and chromatin-remodeler interactions.

UniProtKB:Q9NVR5

Dynein axonemal assembly factor 2 (DNAAF2), Homo sapiens

The reviewed sequence record defines two alternative products of 837 and 789 residues, with an internal 48-residue deletion in product 2.
The curated subunit comment records CFAP300 association with experimental support attributed to PMID:29727693.

file:human/DNAAF2/DNAAF2-hpa.xml

Human Protein Atlas DNAAF2 record, ENSG00000165506, retrieved 2026-10-17

HPA002894 ICC/IF records a basal-body pool in three human cell models with approved gene-level reliability.