Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440
Deep research on mrcA function based on literature review
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Class A PBPs are bifunctional synthases with GT and TP domains
"PBP1A catalyzes (i) GTase activity (EC 2.4.99.28) that polymerizes glycan strands from the lipid II precursor and (ii) Ser-type D,D-TPase activity (EC 3.4.16.4) that crosslinks peptide stems"
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aPBPs require outer membrane lipoprotein activators (LpoA/LpoB/LpoP)
"Requires outer-membrane-anchored lipoprotein activators in Gram-negative bacteria (LpoA for PBP1A in many gamma-proteobacteria)"
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TPase inhibited by beta-lactams, GTase inhibited by moenomycin
"TPase inhibited by beta-lactam antibiotics (penicillins/cephalosporins); GTase inhibited by moenomycin"
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aPBPs contribute to cell envelope mechanical stability
"aPBPs provide mechanical stability (e.g., PBP1B loss decreases stiffness in E. coli)"