FAA1 (YOR317W; UniProt P30624) notes

• Major long-chain acyl-CoA synthetase, EC 6.2.1.3; prefers C12-C16 PMID:8206942.
• Provides most oleoyl-CoA from exogenous oleate PMID:11477098; faa1 faa4 reduces exogenous oleate beta-oxidation 3-fold PMID:11477098.
• Vectorial acylation with Fat1p (co-IP, two-hybrid) PMID:12601005.
• Plasma membrane (GFP) and long-chain base uptake [PMID:27136724 "We found that Faa1-GFP was mainly localized in the plasma membrane"; "These results suggest that Faa1 and Faa4 act as transporters both for LCFAs and LCBs"].
• Lipid droplets and ER [PMID:10515935; PMID:24868093 "Faa1-GFP appeared to be visible in additional cellular compartments, likely the ER"]; mitochondrial proteomics hits are unexplained (non-core).
• Sphingolipid-to-glycerolipid pathway (hexadecenoic acid activation) with Faa4 PMID:22633490.

Pathway-context observations

• YeastPathways places FAA1 at the EC 6.2.1.3 activation step of fatty acid oxidation. Faa1p acts outside peroxisomes; its acyl-CoA reaches beta-oxidation via Pxa1/Pxa2. Contribution real (oleate oxidation reduced in faa1 faa4) but indirect; FAO RCA kept as non-core. Cytosol RCA over-annotated (membrane-associated).