Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins.
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NSG1 and NSG2 stabilize the ER Hmg2 HMG-CoA reductase by directly interacting with its sterol-sensing-domain-containing transmembrane region.
"We now show that the yeast INSIG homologs NSG1 and NSG2 function to control the stability of yeast Hmg2p, the HMGR isozyme that undergoes regulated ubiquitination. Yeast Nsgs inhibit degradation of Hmg2p in a highly specific manner, by directly interacting with the sterol-sensing domain (SSD)-containing transmembrane region."
An in vivo map of the yeast protein interactome.
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.
The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
An inter-species protein-protein interaction network across vast evolutionary distance.
The social and structural architecture of the yeast protein interactome.
Role of Pex31 in metabolic adaptation of the nucleus-vacuole junction.
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NSG1 was identified as a conditional nucleus-vacuole junction resident during glucose limitation, supporting the NVJ localization annotation as context-dependent.
"Here, we used systematic microscopy-based approaches to compare the NVJ at glucose-replete and -restricted conditions and identified five additional NVJ proteins: the permanent NVJ resident Shr5 and the conditional residents Nsg1, Nsg2, Tcb1 and Pex31."
Glucose starvation signaling via nucleus-vacuole junction remodeling controls ergosterol synthesis.
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Glucose starvation recruits Nsg1 and Nsg2 to the nucleus-vacuole junction together with Hmg1 and Hmg2, linking conditional INSIG-family localization to starvation-dependent sterol regulation.
"Here, we identify the aspartyl protease Ypf1 and the yeast INSIG homologs Nsg1 and Nsg2 as NVJ-localized proteins specifically recruited during GS. Ypf1 promotes the recruitment of Nsg1, Nsg2, and the HMG-CoA reductases Hmg1 and Hmg2 to the NVJ, likely in association with changes in nuclear membrane lipid composition caused by suppression of fatty acid elongases."
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Glucose-starvation NVJ remodeling has opposite effects on the two yeast INSIG homologs: it destabilizes Nsg1 to activate Hmg1 while stabilizing Nsg2 to suppress Hmg1, and loss of both proteins hyperactivates Hmg1 and accumulates squalene.
"This remodeling destabilizes Nsg1, thereby activating Hmg1, while stabilizing Nsg2, which suppresses Hmg1 to fine-tune sterol synthesis. Loss of both Nsg1 and Nsg2 leads to hyperactivation of Hmg1 and accumulation of squalene, a sterol biosynthetic intermediate."
Falcon deep research report for NSG1
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The Falcon report was reviewed and synthesized into the NSG1 curation, including core-function framing, family/PANTHER context, and evidence limitations.
Automatic assignment of GO terms using logical inference, based on on inter-ontology links