SGD gene description for BCY1 (yeast)
Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase.
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bcy1 homozygous diploids do not undergo premeiotic DNA replication or form spores.
"In diploids homozygous for the bcy1 mutation that results in deficiency of the regulatory subunit of cAMP-dependent protein kinase and production of a high level of the catalytic subunit of this enzyme, no premeiotic DNA replication and commitment to intragenic recombination occurred, and no spores were formed."
Identification of 14-3-3 proteins, Polo kinase, and RNA-binding protein Pes4 as key regulators of meiotic commitment in budding yeast.
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Meiotic commitment requires full Bcy1 levels, likely by preventing PKA activation.
"Overall, these results demonstrate that meiotic commitment requires full Bcy1 levels, likely by preventing PKA activation."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
PTHR11635 PAINT annotations
UniProtKB entry P07278 for S. cerevisiae BCY1
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UniProt assigns Bcy1 to the PKA regulatory-chain PANTHER family.
"DR PANTHER; PTHR11635; CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN; 1."
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UniProt records Bcy1 interaction with the Tpk1 catalytic subunit.
"CC P07278; P06244: TPK1; NbExp=8; IntAct=EBI-9475, EBI-9458;"
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.
Nucleocytoplasmic distribution of budding yeast protein kinase A regulatory subunit Bcy1 requires Zds1 and is regulated by Yak1-dependent phosphorylation of its targeting domain.
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Bcy1 localization is carbon-source dependent: mostly nuclear in glucose and more evenly split between nucleus and cytoplasm in derepressed cells.
"In glucose-grown cells, Bcy1 is almost exclusively nuclear"
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N-terminal phosphorylation directs Bcy1 toward the cytoplasm.
"phosphorylation of its N-terminal domain directs Bcy1 to the cytoplasm"
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
Subcellular localization of the yeast proteome.
Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast.
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bcy1 deletion was used to rescue ras1 ras2 lethality by bypassing the essential Ras-cAMP pathway.
"the deletion of the BCY1 gene rescues the lethality"
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bcy1 deletion alone did not abolish Lte1 localization to the bud cortex.
"In the Δras1, Δras2, or Δbcy1 cells, Lte1–2HA protein localized properly"
Proteome survey reveals modularity of the yeast cell machinery.
Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.
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Bcy1 appeared as an Ess1 interactor in a WW-domain protein-microarray network.
"the interaction between Ess1 and Bcy1, a regulatory subunit of cAMP-dependent protein kinase A"
The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.
Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast.
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The inactive budding-yeast PKA holoenzyme is composed of two Bcy1 regulatory subunits and two Tpk catalytic subunits.
"PKA is an inactive tetramer composed of two regulatory subunits and two catalytic subunits"
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Krh1/2 promote the Bcy1-Tpk1 association in vivo.
"absence of Krh1/2 reduces the interaction between the catalytic and regulatory subunits of PKA in vivo"
Analysis of the yeast kinome reveals a network of regulated protein localization during filamentous growth.
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Bcy1 becomes predominantly nuclear during filamentous growth.
"The nuclear shift of Bcy1p requires the presence of Ksp1p, Kss1p, and Sks1p."
An in vivo map of the yeast protein interactome.
High-quality binary protein interaction map of the yeast interactome network.
A global protein kinase and phosphatase interaction network in yeast.
The cAMP-dependent protein kinase signaling pathway is a key regulator of P body foci formation.
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae.
The signaling pathways underlying starvation-induced upregulation of α-mannosidase Ams1 in Saccharomyces cerevisiae.
Yeast cAMP-dependent protein kinase can be associated to the plasma membrane.
Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae.
Localization of the regulatory subunit of cAMP-dependent protein kinase in Saccharomyces cerevisiae.
The social and structural architecture of the yeast protein interactome.
Isoform-specific sequestration of protein kinase A fine-tunes intracellular signaling during heat stress.
Characterization of cyclic AMP-requiring yeast mutants altered in the regulatory subunit of protein kinase.