GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

file:PSEPK/PP_0375/PP_0375-uniprot.txt

UniProtKB entry for PP_0375 (prolyl oligopeptidase family protein)

"SubName: Full=Prolyl oligopeptidase family protein"
"DR InterPro; IPR001375; Peptidase_S9_cat."
"DR PANTHER; PTHR43056:SF5; PEPTIDASE S9 PROLYL OLIGOPEPTIDASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1."

file:PSEPK/PP_0375/PP_0375-goa.tsv

QuickGO GOA annotations for PP_0375

"GO:0006508 proteolysis"
"GO:0008236 serine-type peptidase activity"

file:PSEPK/PP_0375/PP_0375-bioinformatics/RESULTS.md

PP_0375 PqqG/PqqM ortholog comparison

"Q88QV9 aligned to AAX10035.1 at 66.0% amino-acid identity across 606 residues, covering 98.9% of Q88QV9 and 99.8% of AAX10035.1."
"The NCBI protein record names AAX10035.1 as the *Pseudomonas fluorescens* B16 `pqqG` gene product, a dipeptidyl aminopeptidase/acylaminoacyl-peptidase."
"B16 full text names `pqqM` among 11 ORFs and states that "the Tn3-gusA insertions in each ORF abolished PQQ production""
"Q88QV9 aligned to the *Pseudomonas aeruginosa* PA1990/PqqH protein Q9I2B9 at 52.2% amino-acid identity across 597 residues, covering 97.1% of Q88QV9 and 98.2% of Q9I2B9."

file:PSEPK/PP_0375/PP_0375-deep-research-openscientist.md

OpenScientist gene research for PP_0375 (Q88QV9)

"PP_0375 is the *P. putida* ortholog occupying the same operonic position."
"The **exact peptide substrate of PP_0375 has not been experimentally established** and remains the principal knowledge gap."

PMID:27287323

Regulation of Pyrroloquinoline Quinone-Dependent Glucose Dehydrogenase Activity in the Model Rhizosphere-Dwelling Bacterium Pseudomonas putida KT2440.

"The pqq gene cluster (pqqFABCDEG) encodes at least two independent transcripts"

PMID:18055583

Pyrroloquinoline quinone is a plant growth promotion factor produced by Pseudomonas fluorescens B16.

"Analysis of the DNA sequence of the fragment identified 11 putative open reading frames, consisting of seven known and four previously unidentified pyrroloquinoline quinone (PQQ) biosynthetic genes."
"All of the pqq genes showed expression only in nutrient-limiting conditions in a PqqH-dependent manner."
"wild-type B16 produces PQQ, whereas mutants defective in plant growth promotion do not"

PMID:19902179

The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa.

"Gene PA1990 of Pseudomonas aeruginosa, located downstream of pqqE and encoding a putative peptidase, was shown to be involved in excretion of PQQ into the culture supernatant."
"The putative pqqF gene of P. aeruginosa was shown to be essential for PQQ biosynthesis."