GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro maps the Importin-beta_N domain (IPR001494) to small GTPase binding and intracellular protein transport. The GTPase binding annotation is appropriate for karyopherin-beta family members, but intracellular protein transport is an over-generalisation for an RNA export receptor.

GO_REF:0000118

TreeGrafter-generated GO annotations

TreeGrafter places B4MAQ2 under PANTHER node PTN000131780 within the EXPORTIN-5 subfamily and transfers RNA binding, RNA export from nucleus, nucleus, cytoplasm and RNA nuclear export complex, consistent with the PAINT IBD placements at PTN000131749/PTN000131750.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined IEA methods assign nuclear export signal receptor activity (consistent with the PAINT family-root IBD, whose GO definition covers RNA cargo) and protein export from nucleus (from ARBA and the combined XPO1/5 InterPro entry; not propagated by PAINT to the exportin-5 clade).

PMID:39098529

Exportin-5 binding precedes 5'- and 3'-end processing of tRNA precursors in Drosophila.

Drosophila Exp5 binds tRNAs (including pre-tRNAs with leader and trailer sequences), miRNA precursors and selected mRNAs/lncRNAs in vivo; Drosophila lacks Exportin-t.

"Consistent with the idea that Exp5 is a major tRNA export receptor, we detected 343 PAR-CLIP clusters identified by Paralyzer at 295 tRNA loci"

PMID:11777942

Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.

Human exportin-5 binds RanGTP specifically, interacts with nucleoporins and shuttles between nucleus and cytoplasm.

"Like other known transport receptors, this Kap binds specifically to RanGTP,"

file:DROVI/B4MAQ2/B4MAQ2-bioinformatics/RESULTS-rereview.md

B4MAQ2 re-review pairwise comparison with characterised exportins

B4MAQ2 aligns end to end with D. melanogaster Ranbp21 (85.9% identity) and is 38.5% identical to human XPO5 versus 25.8% to human XPO1; consistent with a full-length Exportin-5 ortholog.

file:DROVI/B4MAQ2/B4MAQ2-deep-research-falcon.md

Deep research summary for B4MAQ2

Identifies B4MAQ2 as an Exportin-5-like protein based on domain architecture and family classification, with predicted function in RanGTP-dependent nuclear export of structured RNAs.
Notes that in Drosophila, Exportin-5 compensates for the absence of canonical Exportin-t, serving as the major tRNA export factor.
No gene-specific literature exists for Dvir\GJ15622; function is inferred entirely from conserved family and domain assignments.

file:DROVI/B4MAQ2/B4MAQ2-uniprot.txt

UniProt entry B4MAQ2

UniProt classifies the protein in the exportin family with Importin-beta_N, Exportin-1/Importin-b-like, and Exportin-5_C domains.
PANTHER subfamily assignment is EXPORTIN-5 (PTHR11223:SF3), distinguishing it from EXPORTIN-1/CRM1.