GO annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified annotations to orthologs by curator judgment
Annotation inferences using phylogenetic trees
GO annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
GO annotation based on curation of immunofluorescence data (HPA)
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on inter-ontology links
Automatic GO annotation from UniProtKB-KW mapping
Combined Automated Annotation using Multiple IEA Methods
OpenScientist hypothesis investigation - CASP12 cysteine-type endopeptidase activity
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OpenScientist independently judged the IBA GO:0004197 annotation for human CASP12 to be over-annotated and recommended retaining the existing IKR NOT annotation.
"The IBA annotation assigning cysteine-type endopeptidase activity (GO:0004197) to human CASP12 (Q6UXS9) is over-annotated and should be removed or superseded by the existing IKR (Inferred from Key Residues) negation."
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The report concluded that CASP12 acts as a non-catalytic inflammatory regulator rather than as a cysteine endopeptidase.
"Human CASP12 functions as a CARD-only dominant-negative regulator of inflammation, not as an endopeptidase."
Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta.
Human caspase 12 has acquired deleterious mutations.