GA2 (ent-kaurene synthase, KS; At1g79460; UniProt Q9SAK2) - curation notes

• Accession check: Q9SAK2 = KSB_ARATH, gene names GA2/KS/KS1, At1g79460.
• Falcon deep research attempted and failed (exit code 1).

Function

• ent-CPP -> ent-kaur-16-ene + PPi (RHEA:22220, EC 4.2.3.19) PMID:9536043; ga2-1 truncated protein inactive PMID:9536043.
• Single-residue switch converts KS to pimaradiene synthase PMID:17456599.
• Class I cyclase Mg2+ coordination by DDXXD/NSE motifs PMID:21602811.

Location

• GFP targeting to chloroplasts and import into isolated chloroplasts PMID:11722763; chloroplast proteome HDA PMID:18431481.

Curation decisions

• terpene synthase / lyase activity: correct parents of GO:0009899; MODIFY to specific term.
• terpenoid/diterpenoid biosynthesis -> MODIFY to gibberellin biosynthetic process.
• GA signaling TAS: over-annotation.