GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/gloB/gloB-uniprot.txt

UniProtKB reviewed entry for gloB (Q88FF3)

The reviewed record assigns EC 3.1.2.6, Rhea 21864, two zinc ions, and the exact glyoxalase-II family.
Eight HAMAP-derived binding features identify the two catalytic zinc sites.

file:PSEPK/gloB/gloB-goa.tsv

QuickGO GOA annotations for gloB

file:interpro/panther/PTHR43705/PTHR43705-entries.csv

PANTHER PTHR43705 member table

Q88FF3 belongs to the exact PTHR43705:SF1 hydroxyacylglutathione hydrolase subfamily.

PMID:21143325

The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli.

In E. coli, loss of GloB blocks methylglyoxal turnover but does not reproduce the methylglyoxal sensitivity of a gloA deletion.

file:projects/P_PUTIDA/deep-research/PSEPK__methylglyoxal-detoxification__ppu00620-deep-research-openscientist.md

OpenScientist PSEPK methylglyoxal detoxification synthesis

The report identifies PP_4144 as the high-confidence second enzyme in the complete glutathione-dependent route.

file:modules/methylglyoxal_detoxification.yaml

Glutathione-dependent methylglyoxal detoxification module