Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
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The HSI-I locus, which contains PA0091, encodes a T6SS that exports Hcp1.
"Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus."
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
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PA0091 (vgrG1) and PA2685 (then vgrG4, now vgrG1c) are the most abundant proteins of the on-state H1-T6SS secretome; the double deletion abolishes Hcp1 secretion, unlike deletion of the Tse cargo proteins.
"Western blot analysis revealed that Hcp1 secretion was abolished in both the ΔclpV1 and ΔvgrG1 ΔvgrG4 strains, however it was unaffected by tse deletion"
Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins.
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VgrG1a and VgrG1c are secreted in a ClpV1-dependent way, multimerise, and are redundantly required for Hcp1 secretion; VgrG1b behaves differently.
"In conclusion, VgrG1a and VgrG1c are essential for efficient Hcp1 secretion but can function independently."
The structure of VgrG1 from Pseudomonas aeruginosa, the needle tip of the bacterial type VI secretion system.
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The full-length VgrG1a crystal structure is a trimeric needle (head plus beta-roll spike) capping the Hcp1 tube.
"Valine-glycine repeat protein G1 (VgrG1) forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein 1 (Hcp1)"