PANTHER family review PTHR44167: IBA propagation assessment for cds1
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Meiotic DNA replication checkpoint control in fission yeast.
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Cds1 is absolutely required for the meiotic DNA replication checkpoint that blocks meiosis I when replication is incomplete; Cds1 is not required for the mitotic DNA damage checkpoint.
"the Cds1 protein kinase are required for the DNA replication checkpoint during meiosis, with Cds1 playing a more prominent role than it does during mitosis."
Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.
Threonine-11, phosphorylated by Rad3 and atm in vitro, is required for activation of fission yeast checkpoint kinase Cds1.
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Rad3 phosphorylates Cds1 at Thr11 (priming) which is required for HU-induced activation and S-M checkpoint enforcement.
"Substitution of threonine-11 with alanine (T11A) abolished Cds1 activation that occurs when DNA replication is inhibited by hydroxyurea (HU) treatment."
A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway.
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Intra-S-phase slowing of replication after damage depends strongly on Cds1.
"The slowing of S phase depends strongly on the six checkpoint-Rad proteins, on Cds1, and on Rad4/Cut5"
Intra-G1 arrest in response to UV irradiation in fission yeast.
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Cds1 is activated at the onset of S phase after UV and contributes to the intra-S delay; Cds1 kinase activity assayed on MBP.
"Immunoprecipitates of Cds1 were used to measure Cds1 kinase activity, with myelin basic protein (MBP) as substrate"
Two-stage mechanism for activation of the DNA replication checkpoint kinase Cds1 in fission yeast.
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Cds1 is activated in two stages: Mrc1-mediated FHA recruitment and Rad3-dependent priming, then FHA-mediated dimerization and autoactivation.
"Mrc1 recruits Cds1 to stalled replication forks by interactions between the FHA domain of Cds1 and specific phosphorylated Rad3 consensus sites in Mrc1."
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Global GFP localization reports Cds1 in nucleus, cytosol and cell division site.
"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.
DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1.
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Cds1 phosphorylates the MBF repressor Nrm1, promoting its dissociation from MBF and inducing replication/repair gene expression.
"Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its dissociation from the MBF transcription factor."
Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.
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cds1Δ shows a complete S-phase DNA damage checkpoint defect after MMS.
"upon treatment with the alkylating agent methyl methane sulfonate, cds1Delta mutants display a complete checkpoint defect."
Autoinhibition and autoactivation of the DNA replication checkpoint kinase Cds1.
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Autophosphorylation of activation-loop Thr328 is the only modification required for Cds1 kinase activation; the C-terminal tail is autoinhibitory.
"phosphorylation of a highly conserved threonine residue (Thr 328 ) in the activation loop is the only covalent modification required for kinase activation in vitro and in vivo."
The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe.
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Reviews Cds1 (Rad53) as the major replication-checkpoint effector with an SQ/TQ cluster, FHA domain and Ser/Thr kinase domain; primary experimental focus is the paralog Mek1.
"Cds1 and its orthologs in other species ( S. cerevisiae Rad53 and mammalian CHK2) are characterized by an N-terminal Ser-Gln/Thr-Gln (SQ/TQ) cluster and forkhead-associated (FHA) domain and a Ser-Thr kinase domain."
The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing.
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Cds1 phosphorylates Dna2 on S220 in vivo and in vitro to stabilize stalled forks and prevent fork reversal.
"the Cds1(Chk2) effector kinase targets Dna2 on S220 to regulate, both in vivo and in vitro, Dna2 association with stalled replication forks in chromatin."
The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress.
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Cds1 (with Rad24) drives nuclear accumulation of Rgf1 under chronic replication stress via phosphorylation of Cds1-consensus serines.
"Rgf1p nuclear accumulation during replication arrest depends on the 14-3-3 chaperone Rad24p and the DNA replication checkpoint kinase Cds1p."
Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity.
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Cds1 is recruited to chromatin (nucleus); chromatin recruitment depends on CAF-1 subunit Pcf1 and histone H4 acetylation status.
"Chromatin recruitment of Cds1 is enhanced by Pcf1 overproduction but is attenuated by the Δpcf1 mutation."
Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway.
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The Cds1-dependent meiotic replication checkpoint represses mei4 and thereby prolongs horsetail nuclear movement when replication stalls.
"this prolongation was caused by the Cds1-dependent replication checkpoint, which represses expression of the mei4(+) gene encoding a meiosis-specific transcription factor."
Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA.
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The intra-S checkpoint slows replication by inhibiting origin firing (not by decreasing fork rate) and facilitates replication of damaged templates.
"the checkpoint slows replication by inhibiting origin firing, but not by decreasing fork rates. However, the checkpoint appears to facilitate replication of damaged templates, allowing forks to more quickly pass lesions."
A kinase from fission yeast responsible for blocking mitosis in S phase.
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cds1 encodes a protein kinase that is a key component of the DNA replication-monitoring S/G2 checkpoint blocking mitosis during DNA synthesis.
"this protein kinase is a key component of the DNA replication-monitoring S/G2 checkpoint system."
Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1.
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Cds1 (redundantly with Chk1) phosphorylates Cdc25 on S99/S192/S359 to promote 14-3-3 binding as part of the replication checkpoint.
"both kinases phosphorylate Cdc25 on the same sites, which include serine residues at positions 99, 192 and 359."