HFA1 (YMR207C, P32874) notes

• Mitochondrial acetyl-CoA carboxylase paralog of ACC1 PMID:14761959.
• hfa1 null phenocopies mtFAS mutants: no growth on lactate/glycerol, lipoic acid <10% PMID:14761959.
• MTS present and needed; Western blot places Hfa1p in mitochondria PMID:14761959.
• MTS-less HFA1 complements acc1 (artificial cytosolic retargeting) PMID:14761959 -> shows catalytic equivalence, not a native cytosolic role.
• No AUG near 5' end; upstream non-AUG initiation required for function/targeting [UniProt:P32874 CAUTION]. A C-terminal GFP fusion expressed from the annotated ORF may therefore partly mislocalise (possible explanation of cytoplasm HDA).
• YeastPathways places HFA1 in cytosolic FA biosynthesis pathways (PWY3O-10, PWY3O-5962, PWY3O-6336) - compartment wrong; HFA1 belongs to mtFAS (with MCT1, CEM1, OAR1, HTD2, ETR1, ACP1).