UBE2F: exact selected product and same-gene reference

Selected F8WDQ9 has 101 residues; reference Q969M7 has 185 residues.

Biopython PairwiseAligner global BLOSUM62, gap open -10, extension -0.5.

Reference interval Selected interval Identical residues
1–94 1–94 94
179–185 95–101 2

Reference feature mapping (sequence coverage does not establish retained function):

Reference feature Interval Mapped / length Identical
Domain: UBC core 32–185 70 / 154 65
Active site: Glycyl thioester intermediate 116–116 0 / 1 0

A shared segment does not establish that a truncated product folds, is expressed, localizes, or catalyzes the full-length reaction.

Inputs are the sibling frozen ProtNLM source and same-gene reference JSON files. Reproduce with UV_NO_SYNC=1 uv run python compare_sequences.py from this directory.