PA4218 (ampP / ampGh1, Q9HWG8) review notes

MFS permease, 414 aa, 10 TM helices by PhoA/LacZ fusions. Unreviewed UniProt
entry ("Probable transporter"). NCBIfam TIGR02718 sider_RhtX_FptX; TC
2.A.1.57.1; PANTHER PTHR12778 (same family as AmpG). 19% identical to AmpG
(PA4393). Second gene of the ampO-ampP operon (PA4219-PA4218).

Literature (no deep-research provider available; own reading)

Kong et al. 2010 (full text read). Topology: 10 TM helices
PMID:21192796. ampP
mutant lacks maximal beta-lactamase induction and benzylpenicillin induction
of the ampC promoter PMID:21192796. The paper reports no
transport assay; it says only that the topologies are consistent with
transport.
Zhang et al. 2010 (abstract only): ampGh1 (PA4218) deletion had little effect
on resistance and PA4218 does not complement ampG mutants PMID:20713660.
The RhtX/FptX subfamily assignment suggests a siderophore (ferripyochelin)
permease; I could not retrieve the FptX literature (PubMed API errors on
2026-11-05), so the substrate is left open and no siderophore term is
asserted.

PseudoCAP IBA-seed assessment

Other seeds' experimental support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN008542531	GO:0022857 transmembrane transporter activity	4	45 (40 taxa)	GO:0022857, IDA, PMID:21192796 (PseudoCAP)	UNVERIFIABLE	Full text read: topology fusions and induction phenotypes only, no transport assay. The term is plausible on family evidence, but the paper does not show the activity, so the IDA code is wrong.	human SLC33A1 O00400 (IDA), E. coli AmpG P0AE16 (IDA), AmpG PA4393 G3XCT7 (PseudoCAP IMP, SOUND)	Yes; see ampG notes for the node-level judgement

PSEUDOCAP defect: WRONG_EVIDENCE_CODE on the GO:0022857 IDA row, CONFIRMED
(full text read), requested change CHECK (downgrade to ISS or remove). The
term stays accepted on the IBA/IEA rows. No GO_CENTRAL defect; the node-level
assessment is recorded once on ampG.

Other PseudoCAP rows (not seeds)

term	verdict	defect
GO:0009276 Gram-negative-bacterium-type cell wall (IDA)	WRONG	PSEUDOCAP WRONG_TERM, CONFIRMED: the paper's own topology shows an inner-membrane protein; repoint to GO:0005886.
GO:0033252 regulation of beta-lactamase activity (IDA)	WRONG	PSEUDOCAP WRONG_TERM, CONFIRMED: the effect is on ampC promoter induction, not on the enzyme.
GO:0046677 response to antibiotic (IDA)	SOUND-ish, non-core	none (downstream phenotype; companion paper found little effect on resistance).