aguB (PA0293, Q9I6J8) review notes

N-carbamoylputrescine amidohydrolase (EC 3.5.1.53), 292 aa, nitrilase /
carbon-nitrogen hydrolase superfamily (Pfam CN_hydrolase; NCBIfam TIGR03381
agmatine_aguB; InterPro IPR017755), with the Glu-Lys-Cys catalytic triad
(UniProt ACT_SITE 44, 117, 154). Second enzyme of the agmatine route from
arginine to putrescine (AguA agmatine deiminase, then AguB). Unreviewed
UniProt entry.

Literature (no deep-research provider available; own reading)

Haas et al. 1984 (abstract only): agmatine-utilization (agu) mutants;
aguA and aguB were mapped by cotransduction PMID:6423933. This is the PseudoCAP IMP source. The abstract
names aguB as the gene for the enzyme; the IMP most likely rests on loss of
the activity in agu mutants, i.e. a lysate assay, which on its own is weaker
than direct biochemistry.
Nakada & Itoh 2003 (abstract only): purified AguB, specific for
N-carbamoylputrescine PMID:12634339; AguB is a nitrilase-family
protein PMID:12634339; AguA and AguB act biosynthetically in the ADC
route PMID:12634339. This direct biochemistry confirms the term annotated from the
1984 paper.
PubMed MCP search returned API errors on 2026-11-05.

PseudoCAP IBA-seed assessment

Other seeds' experimental support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000557706	GO:0050126 N-carbamoylputrescine amidase activity	2	74 (57 taxa)	GO:0050126, IMP, PMID:6423933 (PseudoCAP)	SOUND	Abstract names aguB as the N-carbamoylputrescine amidohydrolase gene; the activity is independently confirmed with purified AguB (PMID:12634339).	Arabidopsis CPA/NLP1 AT2G27450 (Q8VYF5, IDA PMID:12435743)	Yes; the plant seed has IDA
PTN000557638	GO:0016811 hydrolase activity, acting on C-N (but not peptide) bonds, in linear amides	10	32 (23 taxa)	GO:0050126, IMP, PMID:6423933 (PseudoCAP)	SOUND	GO:0050126 is_a GO:0016811; broad node term suits a nitrilase-superfamily clade.	human, rat, mouse, worm, fly, Dictyostelium, Arabidopsis (x2), Helicobacter seeds	Yes; nine other seeds

No PSEUDOCAP or GO_CENTRAL defect. The 1984 IMP is a genetics-led basis,
but the term is correct for the gene and is supported by later purified-enzyme
kinetics, so I do not ask for it to be changed. Adding an IDA from
PMID:12634339 would strengthen the seed; that is a curation suggestion, not a
defect.