GJB2 manual evidence and source boundaries

Connexin-26 supplies the subunits of an intercellular channel. Its single channel core covers molecular exchange, electrical coupling and structural assembly. Cargo delivery to the surface is a different relationship from doing the delivery.

Selected primary evidence

Source excerpts appear in the review; the separately accessed calcium-wave passage is reproduced here and cited from its annotation. Full-cache availability and actual reading scope are separate: selected original sections are not a claim to have inspected every figure or supplement.

• PMID:12767933(https://pubmed.ncbi.nlm.nih.gov/12767933/): abstract/publisher fragments distinguish human pIRES/EYFP constructs from stable mouse expression.
• PMID:21094651(https://pmc.ncbi.nlm.nih.gov/articles/PMC3026138/), functional Results/Methods: human WT assays used paired Xenopus oocytes and HeLa cells. Plaque imaging includes WT/mutant GFP-4C constructs; M34A/deletion structural preparations are distinct. Conflicting Alexa598/568 labels remain unresolved.
• PMID:16849369(https://pmc.ncbi.nlm.nih.gov/articles/PMC2829448/), Results, “Intercellular calcium waves”: in human WT-expressing HeLa monolayers, mechanical stimulation was “consistently followed by the intercellular spreading of a calcium wave”. Extracellular apyrase controlled ATP-mediated effects. Selected Methods distinguish bicistronic/fused constructs and WT/M34T; this original passage was accessed separately from the abstract-only cache.
• PMID:17551008(https://pubmed.ncbi.nlm.nih.gov/17551008/): selected original text concerns Sf9-expressed human M34A with a C-terminal extension and reconstituted membrane crystals.
• PMID:19340074(https://pubmed.ncbi.nlm.nih.gov/19340074/): authentic abstract and official supplementary text were read, with topology/interface panels inspected. Main Methods were unavailable.
• PMID:26753910(https://pubmed.ncbi.nlm.nih.gov/26753910/), selected Results/Methods: human C211S/C218S-His6 protein was expressed in Sf9 and purified from mixed membranes. Calcium contacts are structural observations; electrostatic permeation effects also rely on computation.

• PMID:19384972(https://pubmed.ncbi.nlm.nih.gov/19384972/): only the authentic abstract was accessible. Tagged WT/variant findings do not identify the uninspected host, tag position or images.

• PMID:17660464(https://pubmed.ncbi.nlm.nih.gov/17660464/), dye-transfer Methods/Results and Figure 4 caption: WT-Cx26-EGFP in HeLa supported Lucifer-yellow/neurobiotin coupling; V84M made plaques without transfer. This is functional channel evidence, separate from promoter-reporter assays. Figure pixels and supplements were not inspected.

• PMID:19864490(https://pubmed.ncbi.nlm.nih.gov/19864490/), Results on consortin perturbation and Methods: mouse recombinant and rat stable-expression Cx26 preparations are distinct. Cx26 is cargo; intracellular accumulation does not establish ERGIC localization. Knockdown did not significantly reduce Cx26 surface plaques. Selected text was read without figure-pixel or supplement inspection.

Interactome and product limits

HuRI, PMID:32296183 supplies 36 incoming partner contexts. The authentic MITAB rows retain P29033 ORF 504 with partial DNA identification. Pooling, array and validated-two-hybrid projections describe stages of the source pipeline, not three independent physiological experiments. Thirty-five literal accession joins are positive; their generic binding labels are uninformative without a demonstrated target mechanism. This does not reject the measured pairs.

Raw context 16 instead carries Q0VAB0 while its candidate source partner is K7ER80/ORF 70162. Both current products are 259 residues but differ at position 136 (C versus R). The retained partial clone identification does not establish equivalence. Its generic annotation is removed as uninformative while the positive gene-level pair remains compatible. A suspected IntAct mapping issue requests a check without asserting a replacement accession; both literal products and the full-clone question are preserved. Official partner FUNCTION/CAUTION scope identifies roles without automatically transferring them to GJB2.

One official human P29033 sequence of 226 residues is established. Substitutions, deletions, fluorescent fusions and purification tags are experimental constructs, not additional natural isoforms. The official record declares no alternative product. Sequence identity and four transmembrane features support the product account; they are not functional assays.

Actual ancestral and ortholog evidence

The retained PTHR11984 book places human P29033 at AN401/PTN002485530. The 948-node tree and 2,873-column alignment were inspected, including four membrane windows. Three ancestral assertion placements govern the six incoming IBAs: AN0/PTN008334405, AN394/PTN000240686 and AN365/PTN002633521. The archive preserves every literal donor group. Target self evidence is legitimate descendant grounding, and donor count is not a quality metric.

The inner-ear-development assertion traces through rat P21994/AN407 to an independent IEP record citing PMID:10570462(https://pubmed.ncbi.nlm.nih.gov/10570462/). Its accessible abstract concerns developmental immunostaining; original Methods and Results remain unavailable. Expression and hearing phenotypes do not resolve which developmental work the product performs. This process remains undecided without alleging an ancestral-placement failure.

The mouse Q00977 record identifies ENSMUSP00000054343 (current version .8) and independently grounded experimental annotations. These are distinct from reciprocal electronic rows. PMID:15692151(https://pubmed.ncbi.nlm.nih.gov/15692151/) was read at abstract level; original construct/species details were not recovered. The donor-record check does not claim full-paper verification.

Curated trafficking context

Reactome R-HSA-451056 maps ERGIC input complex 190641 through entity 190614 to human P29033 residues 1–226; surface output complex 112301 uses entity 53211. Each complex contains six subunits. This is target cargo/location evidence, not a catalytic trafficking role; reaction-level cytosol is not a soluble-protein location.

The other four pathway references provide assembly and delivery context. Cx43-specific overview statements are not transferred to Cx26. The accessible PMID:11719551(https://pubmed.ncbi.nlm.nih.gov/11719551/) abstract distinguishes BFA-resistant traffic from nocodazole-perturbed assembly; the event title does not settle microtubule dependence.

Clinical and emerging context

Reference assessments preserve historical variant interpretations. The M34T conclusion of PMID:14694360 is explicitly marked disputed against PMID:31160754, rather than silently rewritten as a current clinical classification. Papers without an abstract or inspected body remain scientifically unverified despite verified identifiers.

PMID:41514193(https://pmc.ncbi.nlm.nih.gov/articles/PMC12970211/) informs a question, not a new function. Selected original text reports nuclear observations but describes human-cell ChIP alignment to a mouse genome and inconsistent IP-MS sample provenance. These reporting issues require clarification; they do not prove fabrication. ChIP/co-IP and reporter effects do not establish direct sequence-specific DNA contact. No nuclear, DNA-binding or transcription-regulator annotation is proposed.

Incoming-context disposition index

Raw indices refer to the dated exact source objects in the archive. Forecast indices also match the actual native seed exactly.