All 12 source rows, existing Falcon report, actual reference-tree graft and relevant primary transcriptome/proteome results were reviewed. The prior catalytic caution was misinterpreted: PROSITE PRU00121 is named “Kringle domain”; its propagated features are three DISULFID pairs. It is not a catalytic His/Asp/Ser rule. The sequence has TRYPSIN_HIS and TRYPSIN_SER signatures and a complete annotated Peptidase S1 region. Therefore this kringle warning cannot justify withholding all hydrolase/protease functions. It may matter for kringle conformation or cofactor binding, which are distinct issues.
PMID:23411029 full transcriptome/proteome methods and plasminogen-activator section identify a truncated DSPA-gamma product containing kringle and protease domains, with abundant salivary-gland expression/protein. UniProt links this study's JAA47048.1 sequence to K9IJK6. The paper states: “Predicted secondary structure of DSPAγ shows that it is a truncated form displaying only the K1, and protease domains”. This supplies context and inferred identity; it is not a purified K9IJK6 reaction assay. The Falcon narrative mixes human tPA and DSPA-alpha1 domain arrangements, clinical trials and fibrin-selectivity values. Those quantitative alpha1 properties are not assigned to this gamma-like sequence.
Actual TreeGrafter source PTN000667065 is the eutherian tPA reference subtree, below PTN002799995 plasminogen activation/smooth-muscle migration and PTN008611606 PDGFR signaling. The exact bat accession is not a reference-tree leaf; source placement is recovered, not a reconstructed target branch. Broad catalytic and extracellular assertions are retained as supported inference. Alternative salivary specialization does not prove loss of every nonproteolytic tPA role; smooth-muscle migration, PDGFR signaling and the ARBA lipid/oxygen-compound responses remain UNDECIDED for human source/mechanism follow-up. No negative response assay was identified.
Make the molecular work or process role explicit separately for each challenged term; retain evidence-based core versus peripheral judgments rather than treating ontology breadth as non-coreness.
Corrected local-file quotations or matched supporting text to its claim where applicable. Missing-assay caveats remain in reasons rather than serving as positive support. Annotation actions are unchanged.
The focused report supports the existing catalytic and extracellular core for the compact salivary DSPA-gamma protein, but resolves the four pending downstream cell-biology rows as over-annotation. K9IJK6 has kringle and protease domains and lacks the finger/EGF exosites of full-length mammalian tPA; PDGFR signaling, smooth-muscle migration, cellular response to lipid and cellular response to oxygen-containing compound are therefore left out of the core and marked as unsupported carry-overs.