Construction and analysis of photolyase mutants of Pseudomonas aeruginosa and Pseudomonas syringae: contribution of photoreactivation, nucleotide excision repair, and mutagenic DNA repair to cell survival and mutability following exposure to UV-B radiation.
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The cloned PAO1 phr gene complements a photolyase-deficient E. coli strain.
"shown to complement a known phr mutation following expression in Escherichia coli SY2"
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phr mutants have reduced UV-B survival when recovered under photoreactivating light.
"These mutants showed a decrease in survival compared to the wild type of as much as 19-fold after irradiation at UV-B doses of 1,000 to 1,550"
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Survival defect is measured after recovery in photoreactivating light.
"followed by a recovery period under photoreactivating conditions"
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods