file:POPTR/CCOAOMT1/CCOAOMT1-record-evidence.yaml

Compact archived record and sequence evidence for O65862

Dated current records, reference-proteome metadata, exact sequence comparison, and three archived entry versions support the manually assessed alternative-record relationship.

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000002 supplied one or more source GOA annotations reviewed in this file.

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000041 supplied one or more source GOA annotations reviewed in this file.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

GO_REF:0000120 supplied one or more source GOA annotations reviewed in this file.

PMID:10934215

Modifications in lignin and accumulation of phenolic glucosides in poplar xylem upon down-regulation of caffeoyl-coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis.

Down-regulation of poplar CCoAOMT in xylem reduced lignin content and supports a lignin-biosynthesis role for the enzyme.

"Caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) methylates, in vitro, caffeoyl-CoA and 5-hydroxyferuloyl-CoA, two possible precursors in monolignol biosynthesis in vivo."
Reduced CCoAOMT lowers Klason lignin and increases the S/G ratio in xylem.

"the affected transgenic lines had a 12% reduced Klason lignin content, an 11% increased syringyl/guaiacyl ratio in the noncondensed lignin fraction"

file:POPTR/CCOAOMT1/CCOAOMT1-uniprot.txt

UniProtKB reviewed entry for CCOAOMT1

UniProt identifies CCOAOMT1 as caffeoyl-CoA O-methyltransferase EC 2.1.1.104 involved in phenylpropanoid and lignin-related cell wall metabolism.

"FUNCTION: Methylates caffeoyl-CoA to feruloyl-CoA"
CCOAOMT1 also contributes to feruloylated polysaccharide synthesis and cell-wall reinforcement.

"Plays a role in the synthesis of feruloylated polysaccharides."

file:POPTR/CCOAOMT1/CCOAOMT1-deep-research-falcon.md

Falcon deep research report on Populus trichocarpa CCOAOMT1 (O65862)

CCOAOMT1 is a SAM-dependent O-methyltransferase that methylates caffeoyl-CoA in the monolignol/lignin pathway, supplying G-unit precursors.

"placing it in the phenylpropanoid/monolignol pathway where methylation is required for monolignol formation and lignification."
A 2024 synthetic-biology review assigns CCoAOMT the first O-methylation step toward G-type lignin precursors, with COMT acting later toward S-units.

"CCoAOMT performs the first O‑methylation step contributing to G-unit precursor formation"
Because the enzyme is SAM-dependent, cellular methyl-donor balance (SAM:SAH) constrains its flux; SAH inhibits SAM-dependent O-methyltransferases.

"potent inhibitor of SAM-dependent O-methyltransferases, and the"
Antisense down-regulation of CCoAOMT in poplar wood reduced Klason lignin (~12%) and increased S/G (~11%), giving direct Populus evidence for a lignin-biosynthesis role.

"Klason lignin decreased by ~12%"
Reducing this O-methylation step shifts lignin composition in the noncondensed fraction.

"in the noncondensed lignin fraction, indicating that reducing this O-methylation step can shift lignin composition."
2023 multiplex CRISPR editing in P. trichocarpa repeatedly selected CCoAOMT family genes among lignin-pathway targets to redesign wood composition.

"to engineer wood composition for fiber production, with CCoAOMT among the repeatedly selected targets in optimal multigene strategies."
The strongest accessible evidence places CCoAOMT activity in intracellular phenylpropanoid/monolignol metabolism of lignifying xylem cells, though a direct subcellular localization assay was not available.

"the most consistent inference is that PtrCCOAOMT1 operates in the"

file:POPTR/CCOAOMT1/CCOAOMT1-goa.tsv

QuickGO GOA annotations for CCOAOMT1

GOA rows supplied the existing_annotations reviewed in this file.