Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23.
Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family.
Deubiquitination, a new player in Golgi to endoplasmic reticulum retrograde transport.
Proteome survey reveals modularity of the yeast cell machinery.
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme.
Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease.
Reversal of RNA polymerase II ubiquitylation by the ubiquitin protease Ubp3.
High-quality binary protein interaction map of the yeast interactome network.
Cdc48 and Ufd3, new partners of the ubiquitin protease Ubp3, are required for ribophagy.
Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae.
Defining the budding yeast chromatin-associated interactome.
Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress.
Global analysis of yeast mRNPs.
Ras protein/cAMP-dependent protein kinase signaling is negatively regulated by a deubiquitinating enzyme, Ubp3, in yeast.
Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.
Synthetic quantitative array technology identifies the Ubp3-Bre5 deubiquitinase complex as a negative regulator of mitophagy.
The Catalytic Activity of the Ubp3 Deubiquitinating Protease Is Required for Efficient Stress Granule Assembly in Saccharomyces cerevisiae.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
A novel mechanism for the retention of Golgi membrane proteins mediated by the Bre5p/Ubp3p deubiquitinase complex.
The social and structural architecture of the yeast protein interactome.
Falcon deep research report for Saccharomyces cerevisiae UBP3 (Q01477 / YER151C)
-
Ubp3 is a deubiquitinase that catalyzes hydrolysis of the isopeptide bond linking ubiquitin to a substrate lysine, acting through cofactor-controlled recruitment (notably Bre5) rather than as a constitutive housekeeping enzyme.
"Ubp3 catalyzes **deubiquitination**—hydrolysis of the isopeptide bond linking ubiquitin to a substrate lysine"
-
Ubp3 acts in a stable Ubp3·Bre5 complex in which Bre5 is an essential positive regulator of activity, not merely a passive binding partner.
"Bre5 is an essential positive regulator of Ubp3 activity rather than merely a passive binding factor"
-
With Bre5, Ubp3 specifically deubiquitinates the COPII coat subunit Sec23 and is described as the only yeast Ubp able to catalyze Sec23 deubiquitination, anchoring it in ER-to-Golgi anterograde trafficking.
"specifically deubiquitinates the COPII subunit **Sec23**"
-
Ubp3·Bre5 also targets a COPI subunit and BRE5 disruption causes Golgi-to-ER retrograde transport defects, indicating coordination of both anterograde and retrograde ER-Golgi trafficking.
"Disruption of **BRE5** causes defects in Golgi-to-ER retrograde transport"
-
Ubp3-Bre5 is required for starvation-induced ribophagy, removing an inhibitory ubiquitin signal from 60S ribosomal components to permit selective vacuolar delivery.
"Ubp3 is positioned as a DUB that removes an inhibitory ubiquitin signal from ribosomal components"
-
A genome-wide screen identified the Ubp3-Bre5 complex as a negative regulator of rapamycin-induced mitophagy while positively promoting bulk autophagy, ribophagy, and the Cvt pathway.
"negative regulator of rapamycin-induced mitophagy"
-
Additional reported roles include RNA polymerase II deubiquitination, enhancement of proteasomal degradation of sterol-homeostasis enzymes, Rad4 turnover, heat resistance, and replicative lifespan control, plus a 2024 role constraining glucose-mediated mitochondrial repression via phosphate budgeting.
"links Ubp3 to RNAPII deubiquitination, Rad4 proteasomal turnover, heat resistance, and replicative lifespan control"